Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1994-9-27
|
| pubmed:abstractText |
Because prolactin (PRL) plays a role in neonatal immune development, we examined the expression of prolactin receptors (PRL-R) in neonatal lymphoid tissues. We had shown previously that deprivation of milk-borne PRL, days 2-5 in the neonatal rat, leads to enhanced in vitro mitogenesis of thymocytes and splenocytes as well as a change in lymphoid-specific, cell surface antigens (GROVE et al. 1991). In this present study, we asked if neonatal lymphocytes express PRL-R; which forms of PRL-R are expressed (long vs. short form); when these forms are expressed during development; and if milk ingestion plays a role in receptor expression. Two approaches were taken using neonatal rat thymocytes and splenocytes: RNA was analyzed by polymerase chain reaction (PCR) and cells were stained with antibody to PRL-R and analyzed by flow cytometry. In regard to cell surface expression, the percentage of PRL-R positive splenocytes was greater than thymocytes at all ages tested. In the spleen, the percentage of PRL-R positive cells gradually increased to adult levels by day 10; in the thymus the percentage fell to adult levels by the first day after birth. Finally, milk ingestion in the first 7 h decreased the percentage of cells expressing cell surface PRL-R. Tissues from animals deprived of milk during this time expressed PRL-R at the same level as the newborn.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
1210-0668
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
27
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
193-200
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8068896-Administration, Oral,
pubmed-meshheading:8068896-Animals,
pubmed-meshheading:8068896-Animals, Newborn,
pubmed-meshheading:8068896-Base Sequence,
pubmed-meshheading:8068896-Blotting, Southern,
pubmed-meshheading:8068896-Cells, Cultured,
pubmed-meshheading:8068896-Female,
pubmed-meshheading:8068896-Flow Cytometry,
pubmed-meshheading:8068896-Male,
pubmed-meshheading:8068896-Milk,
pubmed-meshheading:8068896-Molecular Sequence Data,
pubmed-meshheading:8068896-Polymerase Chain Reaction,
pubmed-meshheading:8068896-Pregnancy,
pubmed-meshheading:8068896-RNA, Messenger,
pubmed-meshheading:8068896-Rats,
pubmed-meshheading:8068896-Rats, Sprague-Dawley,
pubmed-meshheading:8068896-Receptors, Prolactin,
pubmed-meshheading:8068896-Spleen,
pubmed-meshheading:8068896-Thymus Gland
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
The expression of forms of prolactin receptors in splenocytes and thymocytes of neonatal rats: the effect of milk ingestion.
|
| pubmed:affiliation |
Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|