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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1994-9-29
|
| pubmed:abstractText |
Crystal observation time is a rough estimate of the first microscopic appearance of cholesterol monohydrate crystals in an isotropic bile, and does not provide information on crystal growth kinetics. We have developed a method for quantitating cholesterol crystal growth in gallbladder bile. Crystals were separated from other biliary particles by ultracentrifugation on a discontinuous NaBr gradient, after bile density adjustment to d = 1.060 g/ml. More than 95% of crystals, both of native or synthetic source, floated in the density range 1.045-1.055. This density fraction was collected and crystal mass was measured by photometric turbidity, after calibration with suspensions of different-sized cholesterol crystals. The recovery of crystals added to original bile samples averaged 96.0 +/- 2.8%. Contamination with vesicles, which may potentially interfere with the turbidimetric assay, was excluded by gel-chromatography. The method was sequentially applied, until the 20th day of incubation, to biles obtained at surgery from patients with (A, n = 6) or without cholesterol gallstone (B, n = 4), and from gallstone patients pretreated for 1 week with oral ursodeoxycholic acid (C, n = 5). Crystal growth curves greatly differed, being much steeper in group A and almost flat in patients receiving ursodeoxycholic acid. The mean percent mass of biliary cholesterol in crystalline form at the 20th day was 19.2 +/- 13.5%, 1.2 +/- 0.8% and 2.7 +/- 1.1% in A, B and C, respectively (A vs. B: P = 0.014; A vs. C: P = 0.008). We conclude that the method allows a precise estimate of cholesterol crystal growth and can be usefully applied to human gallbladder biles.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0006-3002
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
1214
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
63-72
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8068730-Bile,
pubmed-meshheading:8068730-Centrifugation, Density Gradient,
pubmed-meshheading:8068730-Chenodeoxycholic Acid,
pubmed-meshheading:8068730-Cholelithiasis,
pubmed-meshheading:8068730-Cholesterol,
pubmed-meshheading:8068730-Crystallization,
pubmed-meshheading:8068730-Gallbladder,
pubmed-meshheading:8068730-Humans,
pubmed-meshheading:8068730-Kinetics,
pubmed-meshheading:8068730-Nephelometry and Turbidimetry,
pubmed-meshheading:8068730-Spectrophotometry,
pubmed-meshheading:8068730-Ursodeoxycholic Acid
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Development and validation of a quantitative assay for cholesterol crystal growth in human gallbladder bile.
|
| pubmed:affiliation |
Division of 2nd Gastroenterology, La Sapienza University, Rome, Italy.
|
| pubmed:publicationType |
Journal Article
|