|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1994-9-27
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|
pubmed:abstractText |
Human Pro-Urokinase (Pro-UK) is expressed in CHO-DHFR- cells at high efficiency by co-transfecting the mouse dhfr gene and Pro-UK cDNA under the control of the SV40 late promoter. After gene co-amplification, the product level could reach 2-3 micrograms/10(6) cells/24 h in the presence of 5 x 10(-6) mol/L MTX, and the levels can be further raised to 3.5-4 micrograms/10(6) cells/24 h by PMA superinduction. The copy number of Pro-UK cDNA in the genomes of host cells is about 200-300 copies/cell. The Western blot analysis shows that the recombinant Pro-UK has similar molecular weight to its natural counterpart, and also the amidolytic activity of the product is determined by S-2444 assay.
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|
pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
1001-652X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
37
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
310-8
|
|
pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8068194-Animals,
pubmed-meshheading:8068194-CHO Cells,
pubmed-meshheading:8068194-Cricetinae,
pubmed-meshheading:8068194-DNA, Complementary,
pubmed-meshheading:8068194-Enzyme Precursors,
pubmed-meshheading:8068194-Gene Amplification,
pubmed-meshheading:8068194-Humans,
pubmed-meshheading:8068194-Recombinant Proteins,
pubmed-meshheading:8068194-Transfection,
pubmed-meshheading:8068194-Urokinase-Type Plasminogen Activator
|
|
pubmed:year |
1994
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|
pubmed:articleTitle |
Gene amplification and high-level expression of human pro-urokinase cDNA in Chinese hamster ovary cells.
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|
pubmed:affiliation |
Laboratory of Genetic Engineering, Molecular Genetics Center, Beijing, PRC.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
