Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-9-21
pubmed:databankReference
pubmed:abstractText
Protein-tyrosine-phosphatases (PTPases) have been implicated in the regulation of certain tyrosine kinase growth factor receptors in that they dephosphorylate the activated (autophosphorylated) form of the receptors. In order to identify PTPases that potentially act on receptor targets in liver, we used the human leucocyte common antigen-related PTPase (LAR) cDNA [Streuli, Krueger, Hall, Schlossman and Saito (1988) J. Exp. Med. 168, 1523-1530] and isolated two closely related transmembrane PTPase homologues from a rat hepatic cDNA library. Both PTPases had large extracellular domains that contained three immunoglobulin-like repeats and eight type-III fibronectin repeats. Both enzymes had tandem homologous PTPase domains following a single hydrophobic transmembrane domain. One sequence encoded the rat homologue of LAR. The second PTPase, designated LAR-PTP2, had 79 and 90% identity with rat LAR in the respective cytoplasmic PTPase domains, with only 57% sequence similarity in the extracellular domain. The catalytic domains of LAR and LAR-PTP2 prepared by bacterial expression were active in dephosphorylating a variety of phosphotyrosyl substrates but did not hydrolyse phosphoserine or phosphothreonine residues of labelled casein. Both enzymes exhibited rapid turnover numbers of 4-7 s-1 for myelin basic protein and 78-150 s-1 for derivatized lysozyme. LAR and LAR-PTP2 displayed similar PTPase activity towards the simultaneous dephosphorylation of receptors of intact insulin and epidermal growth factor from liver membranes. These data indicate that there is a family of LAR-related PTPases that may regulate the phosphorylation state of receptor tyrosine kinases in liver and other tissues.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1280823, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1317540, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1321126, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1323835, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1370625, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1373816, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1375938, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1390871, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1530918, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1547787, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1643113, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1648233, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1648725, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1650499, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1652101, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1655529, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1703321, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1711896, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1719346, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1719347, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1732748, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1736296, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1834176, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1848775, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1852137, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1918076, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-1939050, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2154749, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2157211, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2158859, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2162042, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2164224, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2166945, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2170109, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2294591, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2330003, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2461560, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2468661, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2468674, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2484715, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2546150, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2550143, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2554325, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2824653, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2838265, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-2972792, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-3576199, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-3714490, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-3990573, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-6312838, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-7681589, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-8227050, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-8352946, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-8355697, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-8382771, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-8387037, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-8394171, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-8396131, http://linkedlifedata.com/resource/pubmed/commentcorrection/8068021-8490965
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
302 ( Pt 1)
pubmed:owner
NLM
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