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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1994-9-19
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pubmed:abstractText |
Sixteen Escherichia coli clinical isolates which were resistant to ampicillin and amoxicillin-clavulanate but susceptible to cephalothin were studied. Eight strains showed the presence of a beta-lactamase which comigrates with reference OXA-1 enzyme. The eight other strains produced different TEM-1 derivatives which had in common a higher Km for penicillins and a higher 50% inhibitory concentration for the beta-lactamase inhibitors. By oligotyping and sequencing of PCR products, it was shown that Ser (AGC) (TEM-30; also called TRI-1) in three strains and Cys (TGC) (TEM-31; also called TRI-2) in one strain were substituted for Arg-241 (CGC), that Leu (CTG) (TEM-33) and Val (GTG) (TEM-34) in one strain each were substituted for Met-67 (ATG), and that in other mutants the two latter substitutions occurred together with the substitution of Asp (GAT) (TEM-35 and TEM-36) for Asn-272 (AAT). Therefore, different sets of amino acid substitutions of TEM-1 can be found in clinical isolates and lead to resistance to beta-lactamase inhibitors.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-1316862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-1320587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-1400382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-1490918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-1653204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-1952834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-2039479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-2073111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-2077352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-2118867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-2182386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-2658781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-2687873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-2817854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-2949270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-3015011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-3015017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-3055178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-3107125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-3143303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-3516962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-358200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-6100076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-6272059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-6310323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-6351733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-807678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-8257123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-8392306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8067742-8432315
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0066-4804
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1085-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8067742-Amoxicillin,
pubmed-meshheading:8067742-Base Sequence,
pubmed-meshheading:8067742-Cephalothin,
pubmed-meshheading:8067742-Clavulanic Acid,
pubmed-meshheading:8067742-Clavulanic Acids,
pubmed-meshheading:8067742-Escherichia coli,
pubmed-meshheading:8067742-Escherichia coli Infections,
pubmed-meshheading:8067742-Humans,
pubmed-meshheading:8067742-Kinetics,
pubmed-meshheading:8067742-Microbial Sensitivity Tests,
pubmed-meshheading:8067742-Molecular Sequence Data,
pubmed-meshheading:8067742-Point Mutation,
pubmed-meshheading:8067742-Polymerase Chain Reaction,
pubmed-meshheading:8067742-beta-Lactamases
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pubmed:year |
1994
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pubmed:articleTitle |
Emergence of clinical isolates of Escherichia coli producing TEM-1 derivatives or an OXA-1 beta-lactamase conferring resistance to beta-lactamase inhibitors.
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pubmed:affiliation |
Laboratoire de Microbiologie Médicale, Université Paris VI, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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