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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1994-9-16
|
| pubmed:abstractText |
Neuropeptide Y (NPY) induces contraction of guinea-pig basilar arteries via activation of Y1 receptors. This contraction is blocked by D-myo-inositol-1,2,6-triphosphate (alpha-trinositol). Previous binding studies have shown that alpha-trinositol has no effect at Y1 or Y2 binding sites thus the antagonistic effect should occur at the level of a second messenger. We have examined the effects of NPY on the formation of inositol phosphates (IP) and have looked for an antagonistic effect of alpha-trinositol. NPY (10(-9)-3 x 10-(-7) M) induced strong concentration-dependent contraction of basilar arteries from young guinea-pigs (weight 200-250 g) (Emax: 76.4 +/- 11.1%) but not of arteries from old guinea-pigs (weight > 500 g) (Emax: 2.8 +/- 1.5%). [Pro34]NPY and PYY induced contraction of similar magnitude and potency, whereas NPY13-36 had only a weak effect. This demonstrates an effect via the Y1 type of NPY receptor. The contraction induced by NPY was blocked by alpha-trinositol (p < 0.05). LiCI (2 x 10-4) M), used to inhibit IP breakdown, had no effect on the contraction induced by NPY. NPY (10(-10)-10(-8) M) increased the formation of IP in cerebral vessels from young guinea-pigs from 357 +/- 48 cpm/mg w.w. to 900 +/- 233 cpm/mg w.w. However, there was no alteration in IP formation in cerebral vessels from old guinea-pigs (NPY 10(-9)-10(-7) M). In the presence of alpha-trinositol (10(-8)-10(-6) M) the NPY induced stimulation of IP formation was totally abolished.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0143-4179
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
26
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
305-12
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8065548-Aging,
pubmed-meshheading:8065548-Animals,
pubmed-meshheading:8065548-Basilar Artery,
pubmed-meshheading:8065548-Brain,
pubmed-meshheading:8065548-Guinea Pigs,
pubmed-meshheading:8065548-Inositol Phosphates,
pubmed-meshheading:8065548-Neuropeptide Y,
pubmed-meshheading:8065548-Vasoconstriction
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
alpha-Trinositol blocks neuropeptide Y-induced inositolphosphate formation in cerebral vessels.
|
| pubmed:affiliation |
Department of Experimental Research, General Hospital, Malmö, Sweden.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|