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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1994-9-22
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pubmed:abstractText |
Agonist occupancy of muscarinic cholinergic receptors in human SH-SY-5Y neuroblastoma cells elicited two kinetically distinct phases of phosphoinositide hydrolysis when monitored by either an increased mass of inositol 1,4,5-trisphosphate, or the accumulation of a total inositol phosphate fraction. Within 5s of the addition of the muscarinic agonist, oxotremorine-M, the phosphoinositide pool was hydrolyzed at a maximal rate of 9.5%/min. This initial phase of phosphoinositide hydrolysis was short-lived (t1/2 = 14s) and after 60s of agonist exposure, the rate of inositol lipid breakdown had declined to a steady state level of 3.4%/min which was then maintained for at least 5-10 min. This rapid, but partial, attenuation of muscarinic receptor stimulated phosphoinositide hydrolysis occurred prior to the agonist-induced internalization of muscarinic receptors.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0364-3190
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
549-54
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8065510-Chemical Fractionation,
pubmed-meshheading:8065510-Humans,
pubmed-meshheading:8065510-Hydrolysis,
pubmed-meshheading:8065510-Inositol 1,4,5-Trisphosphate,
pubmed-meshheading:8065510-Neuroblastoma,
pubmed-meshheading:8065510-Neurons,
pubmed-meshheading:8065510-Phosphatidylinositols,
pubmed-meshheading:8065510-Receptors, Muscarinic,
pubmed-meshheading:8065510-Time Factors,
pubmed-meshheading:8065510-Tumor Cells, Cultured
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pubmed:year |
1994
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pubmed:articleTitle |
A rapid attenuation of muscarinic agonist stimulated phosphoinositide hydrolysis precedes receptor sequestration in human SH-SY-5Y neuroblastoma cells.
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pubmed:affiliation |
Department of Pharmacology, University of Michigan Ann Arbor 48104-1687.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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