| pubmed-article:8065330 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8065330 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8065330 | lifeskim:mentions | umls-concept:C0021757 | lld:lifeskim |
| pubmed-article:8065330 | lifeskim:mentions | umls-concept:C0079460 | lld:lifeskim |
| pubmed-article:8065330 | lifeskim:mentions | umls-concept:C0205145 | lld:lifeskim |
| pubmed-article:8065330 | lifeskim:mentions | umls-concept:C0086860 | lld:lifeskim |
| pubmed-article:8065330 | lifeskim:mentions | umls-concept:C0086982 | lld:lifeskim |
| pubmed-article:8065330 | lifeskim:mentions | umls-concept:C2700387 | lld:lifeskim |
| pubmed-article:8065330 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:8065330 | pubmed:dateCreated | 1994-9-19 | lld:pubmed |
| pubmed-article:8065330 | pubmed:abstractText | Granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulates myeloid progenitor cell proliferation and enhances the function of terminally differentiated effector cells. Interleukin-3 (IL-3) stimulation results in the proliferation and maturation of early bone marrow progenitor cells. These activities are mediated by non-tyrosine kinase-containing receptors which consist of ligand-specific alpha subunits that complex with a common beta subunit required for signal transduction. Both GM-CSF and IL-3 rapidly and transiently induce expression of early growth response gene 1 (egr-1) in the human factor-dependent cell line TF-1. To define the mechanism of early response gene induction by GM-CSF and IL-3, growth factor- and serum-starved TF-1 cells transfected with recombinant constructs containing sequences of the human egr-1 promoter were stimulated with GM-CSF or IL-3. A 116-nucleotide (nt) region of the egr-1 promoter which contains sequences inducible by GM-CSF and IL-3 was defined. DNase I footprint analysis identified a 20-nt region, including nt -57 to -76, which contains a potential cyclic AMP (cAMP) response element (CRE). Electrophoretic mobility shift assays performed with CREB antibody confirmed the presence of CREB in the DNA-binding complex. Mutational analysis of the cytokine-responsive region of the egr-1 promoter revealed that both the cAMP response and serum response elements are required for induction by GM-CSF and IL-3. Nuclear extracts from GM-CSF- or IL-3-stimulated but not unstimulated TF-1 cells contain factors which specifically bind to the Egr-1-binding site in the nt -600 to -480 region of the promoter. Electrophoretic mobility shift assays were performed with antibodies against the Egr-1 protein to demonstrate the presence of the protein product in the shifted complex. Our studies suggest that the Egr-1 protein may further stimulate transcription of the egr-1 gene in response to GM-CSF as a secondary event. | lld:pubmed |
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| pubmed-article:8065330 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8065330 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8065330 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8065330 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8065330 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:8065330 | pubmed:issn | 0270-7306 | lld:pubmed |
| pubmed-article:8065330 | pubmed:author | pubmed-author:GassonJ CJC | lld:pubmed |
| pubmed-article:8065330 | pubmed:author | pubmed-author:LeeH JHJ | lld:pubmed |
| pubmed-article:8065330 | pubmed:author | pubmed-author:LehmanEE | lld:pubmed |
| pubmed-article:8065330 | pubmed:author | pubmed-author:SakamotoK MKM | lld:pubmed |
| pubmed-article:8065330 | pubmed:author | pubmed-author:FraserJ KJK | lld:pubmed |
| pubmed-article:8065330 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8065330 | pubmed:volume | 14 | lld:pubmed |
| pubmed-article:8065330 | pubmed:geneSymbol | egr-1 | lld:pubmed |
| pubmed-article:8065330 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8065330 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8065330 | pubmed:pagination | 5975-85 | lld:pubmed |
| pubmed-article:8065330 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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