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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1994-9-19
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pubmed:abstractText |
Granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulates myeloid progenitor cell proliferation and enhances the function of terminally differentiated effector cells. Interleukin-3 (IL-3) stimulation results in the proliferation and maturation of early bone marrow progenitor cells. These activities are mediated by non-tyrosine kinase-containing receptors which consist of ligand-specific alpha subunits that complex with a common beta subunit required for signal transduction. Both GM-CSF and IL-3 rapidly and transiently induce expression of early growth response gene 1 (egr-1) in the human factor-dependent cell line TF-1. To define the mechanism of early response gene induction by GM-CSF and IL-3, growth factor- and serum-starved TF-1 cells transfected with recombinant constructs containing sequences of the human egr-1 promoter were stimulated with GM-CSF or IL-3. A 116-nucleotide (nt) region of the egr-1 promoter which contains sequences inducible by GM-CSF and IL-3 was defined. DNase I footprint analysis identified a 20-nt region, including nt -57 to -76, which contains a potential cyclic AMP (cAMP) response element (CRE). Electrophoretic mobility shift assays performed with CREB antibody confirmed the presence of CREB in the DNA-binding complex. Mutational analysis of the cytokine-responsive region of the egr-1 promoter revealed that both the cAMP response and serum response elements are required for induction by GM-CSF and IL-3. Nuclear extracts from GM-CSF- or IL-3-stimulated but not unstimulated TF-1 cells contain factors which specifically bind to the Egr-1-binding site in the nt -600 to -480 region of the promoter. Electrophoretic mobility shift assays were performed with antibodies against the Egr-1 protein to demonstrate the presence of the protein product in the shifted complex. Our studies suggest that the Egr-1 protein may further stimulate transcription of the egr-1 gene in response to GM-CSF as a secondary event.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-1359492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-1375118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-1455511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-1826565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-1833064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-1845931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-1864967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-1883198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-1898992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-2001448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-2005129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-2052361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-2157471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-2166365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-2166606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-2169613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-2244209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-2461935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-2510170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-2573431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-2652931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-2663885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-2677677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-2687376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-2889129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-2900470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-3025848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-3127059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-3296191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-3821727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-4501582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-6960240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-7504325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-7682176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-7691810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-8114751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-8217192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-8223433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-8319595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-8343951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-8349585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-8373801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-8378773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-8378774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-8397445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-8402883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8065330-8436441
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/EGR1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Early Growth Response Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-3,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:geneSymbol |
egr-1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5975-85
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8065330-Humans,
pubmed-meshheading:8065330-Base Sequence,
pubmed-meshheading:8065330-RNA, Messenger,
pubmed-meshheading:8065330-Binding Sites,
pubmed-meshheading:8065330-Molecular Sequence Data,
pubmed-meshheading:8065330-Cyclic AMP,
pubmed-meshheading:8065330-Structure-Activity Relationship,
pubmed-meshheading:8065330-Gene Expression Regulation,
pubmed-meshheading:8065330-Signal Transduction,
pubmed-meshheading:8065330-Oligodeoxyribonucleotides
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