|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
5
|
|
pubmed:dateCreated |
1994-9-21
|
|
pubmed:abstractText |
The conformational dynamics of the carbohydrate headgroup of ganglioside GD1a, NeuAc alpha 2-->3Gal beta 1-->3GalNAc beta 1-->4[NeuAc alpha 2-->3]Gal beta 1-->4Glc beta 1-->1Cer, anchored in a perdeuterated dodecylphosphocholine micelle in aqueous solution, were probed by high resolution NMR spectroscopy. The observed 1H/1H NOE interactions revealed conformational averaging of the terminal NeuAc alpha 2-->3Gal and Gal beta 1-->3GalNAc glycosidic linkages. The pronounced flexibility of this trisaccharide moiety was substantiated further by two-dimensional proton-detected 13C T1, T1 rho and 1H/13C NOE measurements. The anchoring effect of the micelle allowed the detection of conformational fluctuations of the headgroup on the time scale of a few hundred picoseconds. NMR experiments performed on the GD1a/DPC micelles in H2O at low temperatures permitted the observation of hydroxyl proton resonances, contributing valuable conformational information.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8061213-1420888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8061213-1540698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8061213-185697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8061213-2021640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8061213-2049527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8061213-2110895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8061213-2271623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8061213-2289284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8061213-2306786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8061213-2690953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8061213-4693502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8061213-534624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8061213-534626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8061213-6307308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8061213-8224473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8061213-8448133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8061213-8461305
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0006-3495
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
66
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1642-52
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:8061213-Animals,
pubmed-meshheading:8061213-Biophysical Phenomena,
pubmed-meshheading:8061213-Biophysics,
pubmed-meshheading:8061213-Carbohydrate Conformation,
pubmed-meshheading:8061213-Carbohydrate Sequence,
pubmed-meshheading:8061213-Cattle,
pubmed-meshheading:8061213-Gangliosides,
pubmed-meshheading:8061213-Magnetic Resonance Spectroscopy,
pubmed-meshheading:8061213-Micelles,
pubmed-meshheading:8061213-Models, Molecular,
pubmed-meshheading:8061213-Molecular Sequence Data,
pubmed-meshheading:8061213-Molecular Structure,
pubmed-meshheading:8061213-Phosphorylcholine,
pubmed-meshheading:8061213-Solutions,
pubmed-meshheading:8061213-Thermodynamics,
pubmed-meshheading:8061213-Water
|
|
pubmed:year |
1994
|
|
pubmed:articleTitle |
Carbohydrate dynamics at a micellar surface: GD1a headgroup transformations revealed by NMR spectroscopy.
|
|
pubmed:affiliation |
Complex Carbohydrate Research Center, University of Georgia, Athens 30602.
|
|
pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
|
