Linked Life Data

8059934

Source:http://linkedlifedata.com/resource/pubmed/id/8059934

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-9-15
pubmed:abstractText
In choosing an assay, one needs to consider the following questions: What level of sensitivity is required? Must the assay be continuous? Is the substrate readily available; can it be purchased or must it be custom synthesized? How specific is the substrate? How convenient is the method? How compatible is it with monomolecular, micellar, or vesicular substrates? How tolerant is it of added detergents and proteins that may be present? What is the cost of substrates, fluorescent probes, and instrumentation? Of the many methods described in this review, discontinuous assays using natural substrates and derivatization of the products with fluorescent probes are probably the most reliable and most tolerant of reaction conditions. A drawback is the involvement of tedious and time-consuming steps which limit the number of trials that can be performed. Continuous assays, in which changes in fluorescent properties of the probe are monitored, are most convenient for kinetic studies, although they are also most sensitive to reaction conditions and intolerant of added detergents and proteins. One has to carefully consider all of these issues and choose a method best suited to the enzyme, the particular information one wants to obtain, and the availability of substrates, probes, and instrumentation. Hopefully, increased commercial availability of fluorescent substrates and probes will make these choices easier. Nevertheless, the search goes on for better, more sensitive and convenient fluorescent assays.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
219
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Fluorescence-based assays of lipases, phospholipases, and other lipolytic enzymes.
pubmed:affiliation
Department of Chemistry, St. Olaf College, Northfield, Minnesota 55057.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Review