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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
311
|
pubmed:dateCreated |
1994-9-14
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pubmed:abstractText |
The effect of three different solvents was investigated in run-on experiments, using purified ram sperm nuclei. The best cryoprotective diluent proved to be Nagase-Niwa, which had the most positive effect on transcription. The kinetic data of incorporation of 32P-ATP in pre-mRNA indicated that there was a stepwise increase in the level of transcription during the first 20 min of incubation, being lowest in the control and highest in the presence of the diluent Nagase-Niwa.
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pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:issn |
0011-4529
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
77
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
203-5
|
pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8055705-Animals,
pubmed-meshheading:8055705-Cryopreservation,
pubmed-meshheading:8055705-Cryoprotective Agents,
pubmed-meshheading:8055705-Male,
pubmed-meshheading:8055705-Sheep,
pubmed-meshheading:8055705-Solvents,
pubmed-meshheading:8055705-Spermatozoa,
pubmed-meshheading:8055705-Transcription, Genetic
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pubmed:year |
1994
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pubmed:articleTitle |
The effect of three cryoprotective diluents on the in vitro transcription of ram sperm cells.
|
pubmed:affiliation |
Institute of Biology and Immunology, and Reproduction and Development of Organisms, Bulgarian Academy of Science, Sofia.
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pubmed:publicationType |
Journal Article,
In Vitro
|