Linked Life Data

8053571

Source:http://linkedlifedata.com/resource/pubmed/id/8053571

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-9-7
pubmed:abstractText
In this article a procedure is described which allows us to achieve electroblots of membrane-associated proteins after isoelectric focusing. Proteins are resolved in vertical acrylamide slab gels containing ampholines, urea, and nonionic detergents (dodecyl maltoside and Tergitol NP10). After migration the focusing gel is reinforced by a carrier gel (10% acrylamide) polymerized on only one face of the slab. Electroelution of the proteins toward a hydrophobic membrane (Immobilon P) is obtained by adding denaturing agents to the carrier gel (sodium dodecyl sulfate and mercaptoethanol) and by increasing the methanol concentration (60%) in the transfer buffer. This method is applied to analyzing P450 2A and 3A cytochromes from human liver microsomes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:volume
218
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
80-6
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Electrotransfer of microsomal cytochrome P450 after isoelectric focusing (IEF blots): resolution of human 2A and 3A isozymes.
pubmed:affiliation
Institut National de la Santé et de la Recherche Médicale, Unité 128, BP 5051, Montpellier, France.
pubmed:publicationType
Journal Article