Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1994-9-2
|
| pubmed:abstractText |
Temporal and spatial distribution of a gene encoding murine bone morphogenetic protein 4 (mBMP-4) during fracture repair were investigated in mice by RT-PCR and in situ hybridization. For in situ hybridization, fractured ribs and surrounding tissues were decalcified and hybridized with a mBMP-4-specific complementary RNA probe labeled with digoxigenin-11 UTP. mBMP-4 messenger RNA (mRNA) was not detected in ribs without fracture, whereas it was detected only in the early phase of fracture from 12 to 72 h after the onset of fracture before new cartilage or bone formation. The mBMP-4 mRNAs were present in cells distributed in three distinct regions, namely, the proliferating periosteum, the medullary cavity, and the muscles near the fracture site. These BMP-4-positive cells did not express bone gla protein mRNA, which is a marker of the mature osteogenic cell. RT-PCR also showed a transient increase in the level of BMP-4 mRNA in the early phase of fracture repair. The findings provide us with some new information. (1) The BMP-4 gene is produced by less differentiated osteoprogenitor cells, not by differentiated osteoblasts. (2) The BMP-4 gene is enhanced by the impact of fracture and localized in callus-forming tissue before callus formation. Together with the activities of BMP-4, as was previously described, our results suggest that newly produced BMP-4 gene product is one of the local contributing factors in callus formation in the early phase of fracture healing.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyuracil Nucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Digoxigenin,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/digoxigenin-11-deoxyuridine...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0884-0431
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
651-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8053394-Animals,
pubmed-meshheading:8053394-Base Sequence,
pubmed-meshheading:8053394-Blotting, Southern,
pubmed-meshheading:8053394-Bone Morphogenetic Proteins,
pubmed-meshheading:8053394-Cell Differentiation,
pubmed-meshheading:8053394-Deoxyuracil Nucleotides,
pubmed-meshheading:8053394-Digoxigenin,
pubmed-meshheading:8053394-Fibroblasts,
pubmed-meshheading:8053394-Fracture Healing,
pubmed-meshheading:8053394-Growth Substances,
pubmed-meshheading:8053394-In Situ Hybridization,
pubmed-meshheading:8053394-Male,
pubmed-meshheading:8053394-Mice,
pubmed-meshheading:8053394-Mice, Inbred ICR,
pubmed-meshheading:8053394-Molecular Sequence Data,
pubmed-meshheading:8053394-Osteoblasts,
pubmed-meshheading:8053394-Polymerase Chain Reaction,
pubmed-meshheading:8053394-Protein Biosynthesis,
pubmed-meshheading:8053394-Proteins,
pubmed-meshheading:8053394-RNA, Messenger,
pubmed-meshheading:8053394-Rib Fractures,
pubmed-meshheading:8053394-Stem Cells
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Transient and localized expression of bone morphogenetic protein 4 messenger RNA during fracture healing.
|
| pubmed:affiliation |
Department of Orthopaedic Surgery, Osaka University Medical School, Suita, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|