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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
1994-9-2
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pubmed:abstractText |
Epstein-Barr virus nuclear antigen 2 (EBNA-2) plays an essential role in primary B-lymphocyte growth transformation. EBNA-2 is an acidic transcriptional transactivator that is brought to virus and cell EBNA-2 response elements by interaction with a factor that recognizes the double-stranded sequence MNYYGTGGGAA, where M is A or C, N is any nucleotide, and Y is a pyrimidine. A 63-kDa protein that recognizes this DNA sequence has now been purified by S-Sepharose and oligonucleotide affinity chromatography. p63 peptide sequence is identical to the predicted amino acid sequence for the human J kappa immunoglobulin recombination signal binding protein. Purified or recombinant in vitro-translated J kappa binds to the MNYYGTGGGAA EBNA-2 response element sequence and interacts with EBNA-2. Surprisingly, J kappa does not bind to the J kappa 1 heptamer recombination signal sequence (CACTGTG), and its prior identification as a heptamer binding protein was most likely due to the addition of a BamHI restriction site to the native heptamer creating a near EBNA-2 response element consensus (CACTGTGGGAT).
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-1309714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-1313905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-1328886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-1429518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-1617730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-1649318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-1656076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-1658373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-1740450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-1744127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-1825028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-1849678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-1850028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-2015800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-2352328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-2511556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-2538644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-2547164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-2556266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-2556644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-2556717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-2571937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-3033649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-3047590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-3288540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-3461465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-6816670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-8381349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-8383238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-8391188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-8406481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8052621-8415684
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7568-72
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8052621-Amino Acid Sequence,
pubmed-meshheading:8052621-Antigens, Viral,
pubmed-meshheading:8052621-B-Lymphocytes,
pubmed-meshheading:8052621-Base Sequence,
pubmed-meshheading:8052621-DNA-Binding Proteins,
pubmed-meshheading:8052621-Epithelium,
pubmed-meshheading:8052621-Epstein-Barr Virus Nuclear Antigens,
pubmed-meshheading:8052621-Herpesvirus 4, Human,
pubmed-meshheading:8052621-Molecular Sequence Data,
pubmed-meshheading:8052621-Nuclear Proteins,
pubmed-meshheading:8052621-Protein Binding,
pubmed-meshheading:8052621-Recombinant Proteins,
pubmed-meshheading:8052621-Recombination, Genetic,
pubmed-meshheading:8052621-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8052621-T-Lymphocytes
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pubmed:year |
1994
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pubmed:articleTitle |
The Epstein-Barr virus nuclear antigen 2 transactivator is directed to response elements by the J kappa recombination signal binding protein.
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pubmed:affiliation |
Department of Medicine, Harvard Medical School, Boston, MA 02115.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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