Linked Life Data

8052160

Source:http://linkedlifedata.com/resource/pubmed/id/8052160

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-9-6
pubmed:databankReference
pubmed:abstractText
Tetracycline (pp-tet), and kanamycin (pp-kan) resistance genes were cloned from a transferable R plasmid of fish pathogen Pasteurella piscicida, and complete nucleotide sequences were determined. The pp-tet was a class D Tet determinant constructed with the tetA resistance gene of 1,182 bp encoding a protein with a deduced molecular mass of 41 kDa and the tetR repressor gene of 654 bp encoding a product of 24 kDa. The pp-tet was highly homologous to the tet(D) of plasmid RA1 isolated from Aeromonas hydrophila with two nucleotide differences in the tetR, and of plasmid pIP173 from Salmonella ordonez with two nucleotide differences in the tetA. The pp-kan contained 813 bp encoding a 31 kDa protein of 271 amino acids, and was classified into type aph-Ic. It was identical to the aphA7 in the IAB operon of pBWH77, in which was originally found an isolate of Klebsiella pneumoniae, in its nucleotide sequences and hybrid promoter construction. The genes were connected by an insertion sequence IS26 of 820 bp, and were flanked by repeated copies in direct orientation at the 3' flanking region of the pp-tetA and in inverted orientation at the 3' flanking region of the pp-kan. The genetic elements are organized like a complex transposon by close linkage of the IS26 and the pp-tet and -kan.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0385-5600
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:geneSymbol
aph-Ic, aphA, aphA1, aphA7, kan, tet, tetA, tetR
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
31-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8052160-Amino Acid Sequence, pubmed-meshheading:8052160-Animals, pubmed-meshheading:8052160-Antiporters, pubmed-meshheading:8052160-Bacterial Proteins, pubmed-meshheading:8052160-Base Sequence, pubmed-meshheading:8052160-Cloning, Molecular, pubmed-meshheading:8052160-DNA, Bacterial, pubmed-meshheading:8052160-DNA Transposable Elements, pubmed-meshheading:8052160-Escherichia coli, pubmed-meshheading:8052160-Fish Diseases, pubmed-meshheading:8052160-Fishes, pubmed-meshheading:8052160-Gene Expression Regulation, Bacterial, pubmed-meshheading:8052160-Genes, Bacterial, pubmed-meshheading:8052160-Kanamycin Kinase, pubmed-meshheading:8052160-Kanamycin Resistance, pubmed-meshheading:8052160-Molecular Sequence Data, pubmed-meshheading:8052160-Pasteurella, pubmed-meshheading:8052160-Pasteurella Infections, pubmed-meshheading:8052160-Phosphotransferases (Alcohol Group Acceptor), pubmed-meshheading:8052160-Promoter Regions, Genetic, pubmed-meshheading:8052160-R Factors, pubmed-meshheading:8052160-Repressor Proteins, pubmed-meshheading:8052160-Sequence Alignment, pubmed-meshheading:8052160-Sequence Homology, pubmed-meshheading:8052160-Tetracycline Resistance
pubmed:year
1994
pubmed:articleTitle
The transposon-like structure of IS26-tetracycline, and kanamycin resistance determinant derived from transferable R plasmid of fish pathogen, Pasteurella piscicida.
pubmed:affiliation
Department of Biological Resources, Faculty of Agriculture, Miyazaki University, Japan.
pubmed:publicationType
Journal Article, Comparative Study