Linked Life Data

8050874

Source:http://linkedlifedata.com/resource/pubmed/id/8050874

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-9-8
pubmed:abstractText
Glucose-6-phosphate dehydrogenase (G6PD) was purified from rabbit reticulocytes by using a single immunoaffinity chromatographic step. Antibodies against rabbit erythrocyte G6PD were raised in a goat, purified near to homogeneity and immobilized on CarboLink gel (Pierce). Nonspecific binding sites on the matrix were saturated with myokinase from rabbit muscle. Reticulocyte lysate was directly loaded onto the column, allowed to enter the gel bed and incubated for 15 min at room temperature. The column was washed first with PBS and then with 1 M NaCl. The enzyme was eluted with 0.1M acetic acid in 1M NaCl. Two protein bands of about 66.2 kDa were co-eluted with the G6PD, which was however well separated in SDS-PAGE. The eluent destroyed the enzyme activity but the G6PD yield was higher than 90%.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0021-2938
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
61-70
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Purification of glucose-6-phosphate dehydrogenase from rabbit reticulocytes by immunoaffinity chromatography.
pubmed:affiliation
Istituto di Chimica Biologica G. Fornaini, Università di Urbino.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't