Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1994-9-2
|
pubmed:abstractText |
The effect of acetylcholine on isolated human uterine artery rings was investigated. Acetylcholine induced concentration and endothelium-dependent relaxation (pD2 = 7.29 +/- 0.03) of the precontracted arterial segments. The dissociation constant (KA) for acetylcholine was 1.35 (0.92-1.77) mumol/l. The occupancy-response relationship was non-linear. Half-maximal response to acetylcholine was obtained with 5.25% receptor occupancy. Muscarinic receptor antagonists: atropine, pirenzepine, methoctramine, p-fluoro-hexahydro-sila-diphenidol (pFHHSiD) and 4-diphenyl-acetoxy-N-methyl-piperidine (4-DAMP) competitively antagonized the response to acetylcholine. The constrained pA2 values were 9.32 +/- 0.03, 7.13 +/- 0.01, 6.26 +/- 0.01, 8.17 +/- 0.01 and 9.13 +/- 0.02, respectively. A selective muscarinic M2 receptor antagonist, gallamine, had no effect on acetylcholine-induced relaxation. It is concluded that in human uterine arteries acetylcholine induces endothelium-dependent relaxation and acts as a full agonist. We suggest that the muscarinic receptors involved in the acetylcholine-induced relaxation of the isolated human uterine artery are predominantly of the M3 subtype.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Dibenzylchlorethamine,
http://linkedlifedata.com/resource/pubmed/chemical/Muscarinic Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Papaverine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Muscarinic
|
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0014-2999
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
21
|
pubmed:volume |
256
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
131-9
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:8050463-Acetylcholine,
pubmed-meshheading:8050463-Adult,
pubmed-meshheading:8050463-Arteries,
pubmed-meshheading:8050463-Dibenzylchlorethamine,
pubmed-meshheading:8050463-Endothelium, Vascular,
pubmed-meshheading:8050463-Female,
pubmed-meshheading:8050463-Humans,
pubmed-meshheading:8050463-Middle Aged,
pubmed-meshheading:8050463-Muscarinic Antagonists,
pubmed-meshheading:8050463-Muscle, Smooth, Vascular,
pubmed-meshheading:8050463-Muscle Relaxation,
pubmed-meshheading:8050463-Papaverine,
pubmed-meshheading:8050463-Receptors, Muscarinic,
pubmed-meshheading:8050463-Regional Blood Flow,
pubmed-meshheading:8050463-Uterus
|
pubmed:year |
1994
|
pubmed:articleTitle |
Endothelium-dependent relaxation in response to acetylcholine in the human uterine artery.
|
pubmed:affiliation |
Department of Pharmacology, University Medical Center, Medical Faculty, Belgrade, Yugoslavia.
|
pubmed:publicationType |
Journal Article,
In Vitro
|