Linked Life Data

8048492

Source:http://linkedlifedata.com/resource/pubmed/id/8048492

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1 Pt 1
pubmed:dateCreated
1994-8-30
pubmed:abstractText
Selected enzymes were measured in mixed-fiber bundles and individual fibers from rat plantaris (PL) and soleus (Sol) muscles that had undergone either 2 wk of tetrodotoxin (TTX) inactivation of the sciatic nerve, a sham operation, or were contralateral to the TTX limb. TTX disuse caused severe wasting of PL (46%) and Sol (26%) muscles and of single fibers (50% and 40%, respectively). TTX PL and Sol also had reduced (50%) glycogen content. In TTX, PL, and Sol macro samples and single fibers, the activities (mol.h-1.kg dry wt-1) of hexokinase, glycogen phosphorylase, and lactate dehydrogenase were higher, lower, and unchanged, respectively, compared with controls. Single-fiber data showed that these changes occurred in all fibers. In TTX PL macro samples, activities of glycerol-3-phosphate dehydrogenase (GPDH), pyruvate kinase (PK), malate dehydrogenase (MDH), citrate synthase (CS), beta-hydroxyacyl-CoA dehydrogenase (BOAC), and thiolase were, or tended to be, lower. Single-fiber data showed a disappearance of high-oxidative moderate glycolytic fibers (i.e., usually fast-twitch oxidative in control) and the appearance of more fibers with a metabolic enzyme profile approaching that of control slow-oxidative fibers. In TTX Sol macro samples, GPDH and PK tended to be higher, and thiolase, BOAC, CS, and MDH lower. Single-fiber data corroborated these findings and suggested the appearance of fast fibers with downregulated oxidative enzyme profiles. Our results suggest that neuromuscular activity is a major, but not the sole, determinant of the size and metabolic heterogeneity that exists in muscle cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
267
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C55-66
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Effects of tetrodotoxin-induced neural inactivation on single muscle fiber metabolic enzymes.
pubmed:affiliation
School of Human Movement, Laurentian University, Sudbury, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't