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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
1994-8-30
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pubmed:databankReference | |
pubmed:abstractText |
Sequence comparison of the RNA-dependent RNA polymerases of small round-structured viruses (SRSVs) from 10 recent U.K. outbreaks of gastroenteritis revealed significant genetic variation. Computer analyses indicated that these viruses can be divided into two discrete groups. SRSV group I contains the previously characterized antigenic type 1 Norwalk and type 3 Southampton viruses. The amino acid sequences of the RNA polymerase, capsid and ORF3 of these two viruses are relatively similar (about 92%, 69% and 72% amino acid identity, respectively). A representative member of group II SRSVs, Bristol virus, was subjected to a detailed genetic analysis. Bristol virus is a recent antigenic type 2 isolate from a U.K. hospital outbreak of gastroenteritis. Using a single clinical sample the 3'-terminal 3881 nucleotide cDNA sequence [excluding the poly(A) tail] of this virus was determined. Analysis of the sequence revealed significant differences from those of group I viruses with the RNA polymerase region, capsid and ORF3 showing only about 62%, 43% and 30% amino acid identity respectively with the equivalent proteins of the Norwalk and Southampton viruses. These data suggest that the morphologically identical SRSVs belong to at least two genetically distinct groups.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-1317
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
75 ( Pt 8)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1883-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8046390-Amino Acid Sequence,
pubmed-meshheading:8046390-Base Sequence,
pubmed-meshheading:8046390-Caliciviridae,
pubmed-meshheading:8046390-Capsid,
pubmed-meshheading:8046390-DNA-Directed RNA Polymerases,
pubmed-meshheading:8046390-Gastroenteritis,
pubmed-meshheading:8046390-Genetic Variation,
pubmed-meshheading:8046390-Humans,
pubmed-meshheading:8046390-Molecular Sequence Data,
pubmed-meshheading:8046390-Norwalk virus,
pubmed-meshheading:8046390-Polymerase Chain Reaction,
pubmed-meshheading:8046390-Sequence Analysis, DNA,
pubmed-meshheading:8046390-Sequence Homology, Amino Acid,
pubmed-meshheading:8046390-Serotyping
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pubmed:year |
1994
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pubmed:articleTitle |
Human enteric Caliciviridae: a new prevalent small round-structured virus group defined by RNA-dependent RNA polymerase and capsid diversity.
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pubmed:affiliation |
Department of Molecular Microbiology, University Medical School, Southampton General Hospital, U.K.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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