Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1994-9-1
|
pubmed:abstractText |
The crystal structure of HGPRTase with bound GMP has been determined and refined to 2.5 A resolution. The enzyme has a core alpha/beta structure resembling the nucleotide-binding fold of dehydrogenases, and a second lobe composed of residues from the amino and carboxy termini. The GMP molecule binds in an anti conformation in a solvent-exposed cleft of the enzyme. Lys-165, which forms a hydrogen bond to O6 of GMP, appears to be critical for determining the specificity for guanine and hypoxanthine over adenine. The location of active site residues also provides evidence for a possible mechanism for general base-assisted HGPRTase catalysis. A rationalization of the effects on stability and activity of naturally occurring single amino acid mutations of HGPRTase is presented, including a discussion of several mutations at the active site that lead to Lesch-Nyhan syndrome.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jul
|
pubmed:issn |
0092-8674
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
29
|
pubmed:volume |
78
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
325-34
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:8044844-5'-Guanylic Acid,
pubmed-meshheading:8044844-Amino Acid Sequence,
pubmed-meshheading:8044844-Binding Sites,
pubmed-meshheading:8044844-Crystallography,
pubmed-meshheading:8044844-Crystallography, X-Ray,
pubmed-meshheading:8044844-Humans,
pubmed-meshheading:8044844-Hypoxanthine Phosphoribosyltransferase,
pubmed-meshheading:8044844-Models, Molecular,
pubmed-meshheading:8044844-Molecular Sequence Data,
pubmed-meshheading:8044844-Molecular Structure,
pubmed-meshheading:8044844-Mutation,
pubmed-meshheading:8044844-Protein Conformation,
pubmed-meshheading:8044844-Recombinant Proteins
|
pubmed:year |
1994
|
pubmed:articleTitle |
The crystal structure of human hypoxanthine-guanine phosphoribosyltransferase with bound GMP.
|
pubmed:affiliation |
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|