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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1994-9-1
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pubmed:abstractText |
A limiting-dilution assay was developed and used to determine the frequency of autologous tumor-reactive cytotoxic T lymphocytes (CTL) in peripheral blood of a melanoma patient MZ2, who has been free of detectable disease since several years. In this patient, the frequencies of tumor-reactive CTL spontaneously varied only by a factor of 1.5. After vaccinations with autologous mutagenized and lethally irradiated tumor cells a two- to tenfold increase in frequencies of tumor-reactive CTL was found within the first 2 weeks. Thereafter, CTL frequencies returned to values measured prior to vaccinations. We conclude, that the limiting-dilution assay applied in this study can detect changes in the T cell response to autologous tumor cells. The frequency of tumor-reactive CTL determined with this approach can serve as an immunological parameter for monitoring the T cell response to autologous tumor cells in individual cancer patients receiving tumor cell vaccinations.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0340-7004
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
93-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8044834-Cells, Cultured,
pubmed-meshheading:8044834-Cytotoxicity, Immunologic,
pubmed-meshheading:8044834-Humans,
pubmed-meshheading:8044834-Immunotherapy,
pubmed-meshheading:8044834-Leukocyte Count,
pubmed-meshheading:8044834-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:8044834-Melanoma,
pubmed-meshheading:8044834-Reproducibility of Results,
pubmed-meshheading:8044834-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:8044834-Tumor Cells, Cultured,
pubmed-meshheading:8044834-Vaccination
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pubmed:year |
1994
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pubmed:articleTitle |
Frequency analysis of tumor-reactive cytotoxic T lymphocytes in peripheral blood of a melanoma patient vaccinated with autologous tumor cells.
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pubmed:affiliation |
I. Medizinische Klinik und Poliklinik, Johannes Gutenberg-Universität Mainz, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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