Linked Life Data

8043001

Source:http://linkedlifedata.com/resource/pubmed/id/8043001

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-8-22
pubmed:databankReference
pubmed:abstractText
Four basic neutrophil chemotactic factors (chemokines) have been purified from conditioned medium of granulation tissue obtained from carrageenin-induced inflammation in the rat. On the basis of their N-terminal amino acid sequences, one of the chemokines was identical with rat GRO/cytokine-induced neutrophil chemoattractant (CINC) which we reported previously, and another was identical with rat macrophage inflammatory protein-2 (MIP-2). Two other chemokines were novel chemoattractants related to MIP-2. The novel chemokines are referred to as rat GRO/CINC-2 alpha and CINC-2 beta, and consequently CINC and rat MIP-2 are renamed rat GRO/CINC-1 and CINC-3 respectively. The complete amino acid sequences of purified CINC-2 alpha and CINC-3 were determined by analysis of the fragments isolated from proteinase V8-treated CINCs. The cDNA for CINC-2 beta was cloned by reverse transcription/PCR amplification using specific primers starting with total RNA extracted from lipopolysaccharide-stimulated rat macrophages. A comparison of the amino acid sequence encoded by the cDNA with the N-terminal amino acid sequence of purified CINC-2 beta revealed that mature CINC-2 beta is a 68-residue chemoattractant produced by cleavage of a 32-residue signal peptide. The difference in amino acid sequences between CINC-2 alpha and CINC-2 beta consisted of only three C-terminal residues. Rat GRO/CINC-2 alpha is a major chemokine, and the four purified chemokines have similar chemotactic activity, suggesting that they contribute to neutrophil infiltration into inflammatory sites in rats.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-1337556, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-1415488, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-1420165, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-1547869, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-1548055, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-1592490, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-1639201, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-1744577, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-1884768, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-1910690, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-2026877, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-2182761, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-2201751, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-2217207, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-2244880, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-2449095, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-2662972, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-2671507, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-2677047, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-2684956, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-2824608, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-2890161, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-2970963, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-3258625, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-3260265, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-3322281, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-3480540, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-5778158, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-77677, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-8399143, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-8471066, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-8482545, http://linkedlifedata.com/resource/pubmed/commentcorrection/8043001-8508797
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL1, http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL2, http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC, http://linkedlifedata.com/resource/pubmed/chemical/Chemotactic Factors, http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned, http://linkedlifedata.com/resource/pubmed/chemical/Cxcl1 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Cxcl2 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances, http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides..., http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments, http://linkedlifedata.com/resource/pubmed/chemical/Serine Endopeptidases, http://linkedlifedata.com/resource/pubmed/chemical/glutamyl endopeptidase
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
301 ( Pt 2)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
545-50
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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