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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1994-8-22
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pubmed:abstractText |
At the end of a 12-h day leaves of the mutant of Arabidopsis thaliana L., TC265, contained 4-5 times more starch than those of the wild type. During a subsequent 12-h night the decline in the starch content of the leaves of the mutant was at least 50% of that of the wild-type leaves. Starch labelled in the light in a 30-min pulse in 14CO2 was rapidly broken down in a subsequent 12-h chase in the dark in air in the leaves of both mutant and wild type. Chloroplasts from leaves of the wild type took up [32P]Pi and [U-14C]glucose at 12 and 1.6 mumol/h per mg of chlorophyll respectively; chloroplasts from the mutant showed a similar rate for [32P]Pi but no uptake of [U-14C]glucose. The glucose content of freshly isolated chloroplasts from the mutant was twice that of chloroplasts from the wild type; this difference was accentuated when the isolated chloroplasts were incubated in the dark. SDS/PAGE of preparations of chloroplast envelopes showed that those from the mutant were deficient in a protein band of approximate molecular mass 40 kDa. It is suggested that in mutant TC265 the primary lesion is in a hexose transporter in the chloroplast envelope, and that this transporter moves the products of starch breakdown that are destined for sucrose synthesis from the chloroplast to the cytosol.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
301 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
449-54
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:8042987-Arabidopsis,
pubmed-meshheading:8042987-Biological Transport,
pubmed-meshheading:8042987-Chlorophyll,
pubmed-meshheading:8042987-Chloroplasts,
pubmed-meshheading:8042987-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8042987-Glucose,
pubmed-meshheading:8042987-Monosaccharide Transport Proteins,
pubmed-meshheading:8042987-Mutation,
pubmed-meshheading:8042987-Phosphates,
pubmed-meshheading:8042987-Photoperiod,
pubmed-meshheading:8042987-Starch
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pubmed:year |
1994
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pubmed:articleTitle |
A mutant of Arabidopsis thaliana lacking the ability to transport glucose across the chloroplast envelope.
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pubmed:affiliation |
Department of Plant Sciences, University of Cambridge, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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