Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1994-8-22
|
| pubmed:abstractText |
The cDNAs encoding bovine and porcine interleukin-2 (IL-2) have been expressed using the baculovirus Autographa californica nuclear polyhedrosis virus as a vector in insect cells. Insect cells infected with recombinant viruses secreted bovine and porcine IL-2 into the culture medium, with biological activities for maintaining the proliferation of homologous cells. When the activities of these two IL-2 proteins and commercially available human IL-2 were tested on heterologous cells differences were found. Recombinant bovine (rb)IL-2 only supported the growth of bovine lymphocytes and was not active on human, mouse or porcine lymphocytes. Recombinant porcine (rp)IL-2 and recombinant human (rh)IL-2 supported the proliferation of human, bovine, porcine and murine cells. However, the proliferative response of human lymphocytes to rpIL-2 was only 50% of that seen with rhIL-2. Sequence differences at the predicted p55 and p75 contact binding sites may explain this.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0165-2427
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
40
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
313-24
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8042283-Amino Acid Sequence,
pubmed-meshheading:8042283-Animals,
pubmed-meshheading:8042283-Base Sequence,
pubmed-meshheading:8042283-Cattle,
pubmed-meshheading:8042283-Cells, Cultured,
pubmed-meshheading:8042283-Cloning, Molecular,
pubmed-meshheading:8042283-Cross Reactions,
pubmed-meshheading:8042283-DNA Primers,
pubmed-meshheading:8042283-Gene Expression,
pubmed-meshheading:8042283-Humans,
pubmed-meshheading:8042283-Insects,
pubmed-meshheading:8042283-Interleukin-2,
pubmed-meshheading:8042283-Lymphocyte Activation,
pubmed-meshheading:8042283-Lymphocytes,
pubmed-meshheading:8042283-Molecular Sequence Data,
pubmed-meshheading:8042283-Nucleopolyhedrovirus,
pubmed-meshheading:8042283-Receptors, Interleukin-2,
pubmed-meshheading:8042283-Recombinant Proteins,
pubmed-meshheading:8042283-Sequence Homology, Amino Acid,
pubmed-meshheading:8042283-Species Specificity,
pubmed-meshheading:8042283-Swine
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Cloning and expression of bovine and porcine interleukin-2 in baculovirus and analysis of species cross-reactivity.
|
| pubmed:affiliation |
Division of Immunology and Pathology, Institute for Animal Health, Compton, Newbury, UK.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|