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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1994-8-24
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pubmed:abstractText |
UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8041606-1283705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8041606-1421515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8041606-1554900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8041606-2134343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8041606-2194402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8041606-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8041606-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8041606-7133994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8041606-7688451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8041606-8219322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8041606-8219323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8041606-8291672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8041606-8341593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8041606-8457722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8041606-8481540
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2460-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8041606-Base Sequence,
pubmed-meshheading:8041606-Lasers,
pubmed-meshheading:8041606-Mass Spectrometry,
pubmed-meshheading:8041606-Molecular Sequence Data,
pubmed-meshheading:8041606-Oligodeoxyribonucleotides,
pubmed-meshheading:8041606-Picolinic Acids,
pubmed-meshheading:8041606-Spectrophotometry, Infrared,
pubmed-meshheading:8041606-Spectrophotometry, Ultraviolet,
pubmed-meshheading:8041606-Succinates,
pubmed-meshheading:8041606-Succinic Acid
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pubmed:year |
1994
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pubmed:articleTitle |
Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides.
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pubmed:affiliation |
Institute for Medical Physics and Biophysics, Münster, Germany.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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