Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-8-25
pubmed:abstractText
The aims of this study were (a) to determine if rat alveolar type II (ATII) cells and human pulmonary epithelial-derived cells (A549 cell line) could generate IL-6 in vitro, (b) to characterize the cytokine regulation of IL-6 gene and protein expression in these cells, and (c) to detect the in vivo expression of immunoreactive IL-6 by human ATII cells. Rat ATII cells in primary culture secreted bioactive IL-6 and immunostained with an anti-IL-6 antiserum. Spontaneous IL-6 secretion by rat ATII cells amounted to 5,690 +/- 770 pg/ml/10(6) cells (n = 12) and was fivefold higher than spontaneous rat alveolar macrophages IL-6 secretion (1,052 +/- 286 pg/ml/10(6) cells, n = 8, P = 0.001). Rat alveolar macrophage conditioned media (CM) increased IL-6 secretion by rat ATII cells through the effect of IL-1 and TNF. IL-6 gene expression and IL-6 secretion by A549 cells was induced by IL-1 beta, TNF alpha, and by human alveolar macrophages and THP1 cells CM. Induction was abolished when CM were preincubated with anti-IL-1 beta and anti-TNF alpha antibody. The combination of IFN gamma and LPS induced the expression of IL-6 mRNA by A549 cells whereas LPS alone had no effect. Immunohistochemical staining evidenced the expression of immunoreactive IL-6 by hyperplastic ATII cells in fibrotic human lung, a condition in which alveolar macrophages are known to be activated. ATII cells in normal human lung did not express immunoreactive IL-6. Our findings demonstrate that ATII cells may be an important source of IL-6 in the alveolar space thereby participating to the regulation of the intra-alveolar immune response.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-1316730, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-1396973, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-1415725, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-1478679, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-1554202, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-1591014, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-1647416, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-1868982, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-1892646, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-1910808, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-1918983, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-1958380, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-1988228, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2024703, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2033076, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2045425, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2123224, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2127356, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2159222, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2170444, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2185652, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2199284, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2221080, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2223104, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2254454, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2294996, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2358670, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2462386, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2472425, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2472956, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2500051, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2649105, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2732467, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2790194, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-2966814, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-326115, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-3491322, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-3500054, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-3706902, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-3771800, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-6606682, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-6732053, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-7015935, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-7054589, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-7103258, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-7406333, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-7679411, http://linkedlifedata.com/resource/pubmed/commentcorrection/8040328-8476068
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9738
pubmed:author
pubmed:issnType
Print
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
731-40
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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