Linked Life Data

8040005

Source:http://linkedlifedata.com/resource/pubmed/id/8040005

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1994-8-25
pubmed:abstractText
Endo-beta-galactosidase from Escherichia freundii cleaves polylactosaminyl structures as follows: R-GlcNAc beta I-3Gal beta I-4GlcNac beta I-R' + H2O-->R-GlcNAc beta I-3Gal + GlcNAc beta I-R'. By staining with Griffonia simplicifolia agglutinin-II following the enzyme digestion, the distribution of R-GlcNAc beta I-3Gal beta I-4GlcNAc can be demonstrated in tissue sections. This carbohydrate chain is one of the backbone structures carrying the blood-group-related antigens and, thus, localization of this structure may provide detailed information about the distribution of variants with different backbone structures. Various formalin-fixed, paraffin-embedded tissue sections were stained by Griffonia simplicifolia agglutinin-II with or without prior enzyme digestion and the reactivity of the agglutinin imparted by enzyme digestion was studied in the following tissues and cells: pancreatic acinar cells, gastric surface mucosae, duct cells and mucous cells of salivary glands and tracheal glands, surface epithelium of trachea, goblet cells of large intestine, columnar epithelium of uterine cervical glands, distal and collecting tubules of kidney, certain cells of anterior lobe and colloid of middle lobe of pituitary glands, epithelial reticular cells and Hassall's corpuscles of thymus and Kupffer cells of liver. In gastric surface mucosae, the reactivity of the agglutinin appeared in non-secretor individuals but not in the secretor individuals, and in mucous cells of salivary and tracheal glands the reactivity appeared in Le(a- b-) non-secretor individuals but not in Le(a + b-) non-secretor or secretor individuals.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0018-2214
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
327-36
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8040005-Adult, pubmed-meshheading:8040005-Blood Group Antigens, pubmed-meshheading:8040005-Carbohydrate Sequence, pubmed-meshheading:8040005-Digestive System, pubmed-meshheading:8040005-Female, pubmed-meshheading:8040005-Glycoside Hydrolases, pubmed-meshheading:8040005-Histocytochemistry, pubmed-meshheading:8040005-Humans, pubmed-meshheading:8040005-Kidney Tubules, pubmed-meshheading:8040005-Lectins, pubmed-meshheading:8040005-Male, pubmed-meshheading:8040005-Molecular Sequence Data, pubmed-meshheading:8040005-Pituitary Gland, pubmed-meshheading:8040005-Plant Lectins, pubmed-meshheading:8040005-Polysaccharides, pubmed-meshheading:8040005-Submandibular Gland, pubmed-meshheading:8040005-Thymus Gland, pubmed-meshheading:8040005-Tissue Distribution, pubmed-meshheading:8040005-Uterus, pubmed-meshheading:8040005-beta-Galactosidase
pubmed:year
1994
pubmed:articleTitle
Localization of blood-group-related linear poly-N-acetyllactosamine structure in different human tissues by Griffonia simplicifolia agglutinin-II staining following endo-beta-galactosidase digestion.
pubmed:affiliation
Department of Legal Medicine, Nara Medical University, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't