|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
8
|
|
pubmed:dateCreated |
1994-8-24
|
|
pubmed:abstractText |
The glurp gene of Plasmodium falciparum F32 has been inserted into a vaccinia virus, and the recombinant virus was designated VVG4. Expression of glurp in VVG4-infected Vero cells was analyzed by immunoprecipitation and revealed a primary GLURP product of approximately 220,000 Da; GLURP was detected both intracellularly and in culture supernatants. To study the immunogenicity of vaccinia virus-expressed GLURP, mice were immunized with VVG4 and serum samples were analyzed for antibody reactivity with three polypeptides, covering almost the entire GLURP molecule; these three polypeptides were produced in recombinant form in Escherichia coli. The immune response was primarily directed against a carboxy-terminal repeat region. The mouse anti-GLURP serum recognized authentic GLURP by immunoprecipitation analysis from P. falciparum grown in vitro. These results demonstrate that vaccinia virus-expressed glurp product can induce a humoral immune response against GLURP derived from blood-stage parasites.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-1431149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-1547959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-1558270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-1711015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-1775153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-1779992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-1925544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-2035752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-2457214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-2715642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-3056855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-6546423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-7685119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-781840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-8359915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-8381248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-8418032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8039897-8458956
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0019-9567
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
62
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
3270-5
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:8039897-Animals,
pubmed-meshheading:8039897-Base Sequence,
pubmed-meshheading:8039897-Female,
pubmed-meshheading:8039897-Humans,
pubmed-meshheading:8039897-Malaria Vaccines,
pubmed-meshheading:8039897-Mice,
pubmed-meshheading:8039897-Mice, Inbred BALB C,
pubmed-meshheading:8039897-Molecular Sequence Data,
pubmed-meshheading:8039897-Molecular Weight,
pubmed-meshheading:8039897-Plasmodium falciparum,
pubmed-meshheading:8039897-Protozoan Proteins,
pubmed-meshheading:8039897-Protozoan Vaccines,
pubmed-meshheading:8039897-Vaccines, Synthetic,
pubmed-meshheading:8039897-Vaccinia virus
|
|
pubmed:year |
1994
|
|
pubmed:articleTitle |
Immunogenicity of the Plasmodium falciparum glutamate-rich protein expressed by vaccinia virus.
|
|
pubmed:affiliation |
Department of Infection-Immunology, Statens Seruminstitut, Copenhagen, Denmark.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
