Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1994-8-25
pubmed:abstractText
The possibility of using polymorphisms in the spacer regions between 16S and 23S rRNA genes in order to type Staphylococcus aureus has been evaluated. To this purpose, DNA extracted from 74 independent isolates was amplified making use of a pair of primers complementary to conserved regions in the 16S and 23S genes. We have demonstrated that the method provides a good discrimination between unrelated isolates, giving better results when methicillin-sensitive strains are considered. Moreover, the amplification profiles were reproducible and all strains were typable. Given these results, and the technical simplicity of the process, we propose PCR-ribotyping to be taken into consideration as a method for typing S. aureus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0378-1097
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
119
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
167-73
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Typing of Staphylococcus aureus by amplification of the 16S-23S rRNA intergenic spacer sequences.
pubmed:affiliation
Istituto di Microbiologia, Università degli Studi, Trieste, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't