Linked Life Data

8039171

Source:http://linkedlifedata.com/resource/pubmed/id/8039171

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-8-24
pubmed:abstractText
The heterogeneous nature of most polysaccharides found in nature includes distribution in molecular weight, primary sequence, and branching. The analytical methodology used in the characterization of these structural aspects must ensure high separation efficiency and selectivity. This paper reports on the high-performance capillary electrophoresis (HPCE) separation of branched forms of oligosaccharides as well as some variants in the primary structure. Oligosaccharide maps were obtained after selective debranching using isoamylase, laminarinase, and cellulase enzymes. The samples investigated were alpha-D-glucans (amylose, amylopectin, and pullulan) and beta-D-glucans (exemplified by lichenan). The solutes were separated as fluorescent derivatives with 8-aminonaphthalene-1,3,6-trisulfonate (ANTS) and detected by laser-induced fluorescence at 514 nm using a He/Cd laser (excitation at 325 nm). The number of theoretical plates was in excess of one million per meter. Baseline resolution of oligosaccharides with a degree of polymerization approximately 70 was obtained within 15 min using borate buffer as the electrolyte.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0008-6215
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
258
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Resolution of the branched forms of oligosaccharides by high-performance capillary electrophoresis.
pubmed:affiliation
Department of Chemistry, Indiana University, Bloomington 47405.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't