Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-8-23
pubmed:databankReference
pubmed:abstractText
The E. coli chromosome is known to carry at least five genes, each of which codes for a "D-E-A-D" box protein that is presumed to possess an ATP-dependent RNA helicase activity. Four of such genes (srmB, deaD, dbpA and rhlB) were already mapped on the E. coli chromosome and their DNA sequences determined. We here report the complete nucleotide sequence of the remaining rhlE gene located at about 17.8 min on the E. coli genetic map. RhlE protein possesses all of the motifs (I to VI) conserved among prokaryotic and eukaryotic "D-E-A-D" proteins and has an arginine-rich carboxyl-terminal region. A null mutant of the rhlE gene was constructed by a new method with a ColE1 plasmid mutant that replicates in RNAse HI-deficient bacterial strains, but not in the wild-type strains. The delta rhlE mutant can grow normally, implying that the rhlE gene product is nonessential for bacterial cell growth.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-504X
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:geneSymbol
rhlE
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Structural analysis of the rhlE gene of Escherichia coli.
pubmed:affiliation
Institute for Virus Research, Kyoto University, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't