Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-8-16
pubmed:abstractText
Protein disulphide isomerase (PDI) was shown to be able to accelerate the refolding of unfolded recombinant prochymosin and to enhance the overall yield of active protein. Unlike previous reports in this study PDI was found to be active at pH values as high as 11. The coincidence of the similar apparent optimum pH values of uncatalysed and PDI-catalysed reactions suggests that conditions favourable to spontaneous refolding of proteins may help PDI to catalyse thiol/disulphide interchange. Under the conditions described here no exogenously added dithiothreitol was required for PDI-catalysed renaturation, implying that the disulphide form of PDI was reduced to its active form by the free thiol groups in prochymosin molecules.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8037666-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/8037666-2025220, http://linkedlifedata.com/resource/pubmed/commentcorrection/8037666-2025221, http://linkedlifedata.com/resource/pubmed/commentcorrection/8037666-2025222, http://linkedlifedata.com/resource/pubmed/commentcorrection/8037666-2241930, http://linkedlifedata.com/resource/pubmed/commentcorrection/8037666-2619767, http://linkedlifedata.com/resource/pubmed/commentcorrection/8037666-3548705, http://linkedlifedata.com/resource/pubmed/commentcorrection/8037666-5330666, http://linkedlifedata.com/resource/pubmed/commentcorrection/8037666-6304731, http://linkedlifedata.com/resource/pubmed/commentcorrection/8037666-6503714, http://linkedlifedata.com/resource/pubmed/commentcorrection/8037666-6530042, http://linkedlifedata.com/resource/pubmed/commentcorrection/8037666-7431409
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
301 ( Pt 1)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17-20
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Assisted refolding of recombinant prochymosin with the aid of protein disulphide isomerase.
pubmed:affiliation
Institute of Microbiology, Chinese Academy of Sciences, Beijing.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't