Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1994-8-12
|
| pubmed:abstractText |
Cytogenetic analysis of a pediatric patient with T-cell acute lymphoblastic leukemia (T-ALL) revealed a mosaic karyotype, 47,XX,+17,t(11;14)(p13;q11)/47,XX,+17,t(9;22)(q34;q11),t(11;14) (p13;q11). DNA blot analysis was used to examine the break-point within the BCR gene on chromosome 22 and showed that the breakpoint occurred within the 20-kb minor breakpoint cluster region (m-bcr) located within the first intron of the BCR gene. Immunoprecipitation analysis demonstrated that the leukemic cells expressed the P185 BCR-ABL protein tyrosine kinase. P185 BCR-ABL has previously been shown to be expressed in most cases of Ph+ acute leukemia of myeloid and B-progenitor origin. Here, we demonstrate for the first time that P185 can also be expressed in the T-cell lineage. DNA blot hybridization was also used to characterize the t(11;14) translocation. This showed rearrangement on chromosome 11 within the T-ALLbcr region, upstream of the RBTN-2 gene. Polymerase chain reaction revealed the presence of RBTN-2 transcripts in the leukemic cells. Finally, comparison of the T-ALLbcr, BCR-ABL, IGH, TCR beta and gamma gene rearrangements in leukemic cells obtained at the time of diagnosis and at first relapse showed that relapse occurred in a leukemic clone indistinguishable from the major Ph+ clone involved at diagnosis. Together, these data support a multistep pathogenesis in which the Philadelphia (Ph) chromosome translocation appeared subsequent to the +17 and t(11;14) and imparted a growth advantage over the Ph-negative cells that carried these abnormalities.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/LIM Domain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/LMO1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0887-6924
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
8
|
| pubmed:geneSymbol |
IGH,
RBTN-2
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1124-30
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8035604-Base Sequence,
pubmed-meshheading:8035604-Child,
pubmed-meshheading:8035604-Chromosomes, Human, Pair 11,
pubmed-meshheading:8035604-Chromosomes, Human, Pair 14,
pubmed-meshheading:8035604-DNA, Neoplasm,
pubmed-meshheading:8035604-DNA-Binding Proteins,
pubmed-meshheading:8035604-Female,
pubmed-meshheading:8035604-Gene Expression,
pubmed-meshheading:8035604-Genes, abl,
pubmed-meshheading:8035604-Humans,
pubmed-meshheading:8035604-Immunoblotting,
pubmed-meshheading:8035604-Immunophenotyping,
pubmed-meshheading:8035604-Karyotyping,
pubmed-meshheading:8035604-LIM Domain Proteins,
pubmed-meshheading:8035604-Leukemia-Lymphoma, Adult T-Cell,
pubmed-meshheading:8035604-Molecular Sequence Data,
pubmed-meshheading:8035604-Oncogene Proteins,
pubmed-meshheading:8035604-Philadelphia Chromosome,
pubmed-meshheading:8035604-RNA, Neoplasm,
pubmed-meshheading:8035604-Transcription Factors,
pubmed-meshheading:8035604-Translocation, Genetic
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Simultaneous expression of RBTN-2 and BCR-ABL oncogenes in a T-ALL with a t(11;14)(p13;q11) and a late-appearing Philadelphia chromosome.
|
| pubmed:affiliation |
Department of Human Oncology, University of Wisconsin, Madison 53792.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Case Reports,
Research Support, Non-U.S. Gov't
|