Linked Life Data

8035230

Source:http://linkedlifedata.com/resource/pubmed/id/8035230

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1994-8-16
pubmed:abstractText
1. The effects of the di- and trivalent trace metal cations, Pb2+, Zn2+, and Al3+, on voltage-activated calcium channel currents recorded from cultured rat dorsal root ganglion neurons were determined. 2. All three cations blocked transient and sustained components of the voltage-gated calcium channel currents elicited by a voltage jump from -80 mV to 0 mV, but the trace metals differed in threshold, reversibility, and specificity, and in actions on the different components. 3. Pb2+ was most effective in reducing the voltage-activated calcium channel currents. Threshold concentration for Pb2+ was < 0.1 microM. The threshold for Zn2+ action was < 5 microM and that for Al3+ was approximately 20 microM. Total blockade (> 80%) was obtained with concentrations > 1 microM Pb2+, and 150-200 microM Zn2+ or Al3+. Half of the current was blocked with 0.6 microM Pb2+, 69 microM Zn2+, and 84 microM Al3+. The Hill slope for Pb2+ and Zn2+ action was approximately 1, whereas for Al3+ it was close to 3. 4. Al3+ blockade was clearly use dependent, whereas this was not the case for either Pb2+ or Zn2+. 5. The blockade by none of these metals was totally reversible. The best recovery was obtained upon wash after exposure to Pb2+ (> or = 60%), some recovery was seen with Zn2+ (> or = 50%), but there was little or no recovery after application of Al3+. 6. With Zn2+ or Al3+ in the external solution the current-voltage relation often shifted to depolarized voltages.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-3077
pubmed:author
pubmed:issnType
Print
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1491-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Mammalian voltage-activated calcium channel currents are blocked by Pb2+, Zn2+, and Al3+.
pubmed:affiliation
Heinrich Heine Universität Düsseldorf, Physiologisches Institut II, Germany.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't