Linked Life Data

8031929

Source:http://linkedlifedata.com/resource/pubmed/id/8031929

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-8-12
pubmed:abstractText
The spectral behaviour of calcein, a water-soluble self-quenching molecule that is often used as a marker in biomedical analysis, can be considerably affected by the presence of surfactants. The aim of this work was to investigate the spectral properties of calcein under conditions that are particularly significant in liposome studies. For this purpose the fluorescence and absorbance of this dye were determined in solutions of ionic and non-ionic surfactants (cetyltrimethylammonium bromide, sodium dodecyl sulphate and Triton X-100), at different concentrations below and above their critical micelle concentrations (c.m.c.) as well as in the presence of phospholipids in the form of liposomes and/or mixed micelles. Cationic surfactants induced changes in lambda max, absorbance and fluorescence but these changes were less noticeable in the phospholipid dispersions. The anionic and the non-ionic surfactants induced mainly changes in fluorescence intensity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0731-7085
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
307-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Effects of surfactants on the spectral behaviour of calcein.
pubmed:affiliation
Dipartimento di Studi di Chimica e Tecnologia delle Sostanze Biologicamente Attive, Università La Sapienza, Rome, Italy.
pubmed:publicationType
Journal Article