Linked Life Data

8031765

Source:http://linkedlifedata.com/resource/pubmed/id/8031765

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
27
pubmed:dateCreated
1994-8-16
pubmed:abstractText
Different denatured states of Arc repressor were characterized by one-dimensional and two-dimensional NMR and by fluorescence spectroscopy. Increasing pressure promoted sequential changes in the structure of Arc repressor: from the native dimer through a predissociated state to a denatured molten globule monomer. A compact state (molten globule) of Arc repressor was obtained in the dissociation of Arc repressor by pressure whereas high temperature and urea induced dissociation and unfolding to less structured conformations. The NMR spectra of the monomer under pressure (up to 5.0 kbar) are typical of a molten globule, and they are considerably different from those of the native dimer and the thermally or chemically denatured monomer. The substantial line broadening and overlap of many resonances in the NMR spectra at high pressures indicate that there is interconversion between a number of different conformations of the molten globule at an intermediate exchange rate. The two-dimensional NOE spectra show that the pressure-denatured monomer retains substantial secondary structure. The presence of NOEs in the beta-sheet region in the dissociated state suggests that the intersubunit beta-sheet (residues 6-14) in the native-dimer is replaced by an intramonomer beta-sheet. Changes in 2D NMR spectra prior to dissociation indicate the existence of a predissociated state that may represent an intermediate in the folding and subunit association pathway of Arc repressor.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8323-9
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
High-pressure NMR study of the dissociation of Arc repressor.
pubmed:affiliation
School of Chemical Sciences, University of Illinois, Urbana 61801.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't