Linked Life Data

8027997

Source:http://linkedlifedata.com/resource/pubmed/id/8027997

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-8-11
pubmed:abstractText
The crystal structure of Fab17-IA, an antigen-binding fragment from a murine immunoglobulin that neutralizes human rhinovirus 14 (HRV14), has been solved to 2.7 A resolution. Fab17-IA crystallized into three different space groups depending upon the method used to purify the intact antibody. The structure was determined by use of molecular and isomorphous replacement methods. The current model has a crystallographic R-factor of approximately 19% for 10,192 independent reflections between 8 and 2.7 A. Correlation coefficient calculations showed that the Fab17-IA structure can be fit into the Fab17-IA/HRV14 image reconstruction density to within 5 A positional accuracy and to within a few degrees of rotation. The resulting interface of the docked antibody was examined and showed extensive charge and shape complementarity with the virus surface that was supported by site-directed mutagenesis experiments. The success of this approach validates the utility of combining X-ray crystallography with cryo-electron microscopy of complex macromolecular assemblies.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-2836
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
240
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
127-37
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8027997-Amino Acid Sequence, pubmed-meshheading:8027997-Antibodies, Monoclonal, pubmed-meshheading:8027997-Antibodies, Viral, pubmed-meshheading:8027997-Antigen-Antibody Complex, pubmed-meshheading:8027997-Base Sequence, pubmed-meshheading:8027997-Cryopreservation, pubmed-meshheading:8027997-Crystallography, X-Ray, pubmed-meshheading:8027997-Humans, pubmed-meshheading:8027997-Immunoglobulin Fab Fragments, pubmed-meshheading:8027997-Microscopy, Electron, pubmed-meshheading:8027997-Models, Molecular, pubmed-meshheading:8027997-Molecular Sequence Data, pubmed-meshheading:8027997-Neutralization Tests, pubmed-meshheading:8027997-Protein Conformation, pubmed-meshheading:8027997-Rhinovirus, pubmed-meshheading:8027997-Sequence Homology, Amino Acid, pubmed-meshheading:8027997-Structure-Activity Relationship
pubmed:year
1994
pubmed:articleTitle
Structure determination of an Fab fragment that neutralizes human rhinovirus 14 and analysis of the Fab-virus complex.
pubmed:affiliation
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't