Linked Life Data

8025673

Source:http://linkedlifedata.com/resource/pubmed/id/8025673

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-8-10
pubmed:abstractText
By differential hybridization we have identified cDNA clones that are derived from iron-regulated genes in Saccharomyces cerevisiae. Sequencing of seven cDNA clones revealed that five clones correspond to TPI1 encoding triosephosphate isomerase (Tpi1p) and one corresponds to TDH3 encoding glyceraldehyde-3-phosphate dehydrogenase (Tdh3p). During iron-limited growth mRNA levels for Tpi1p and Tdh3p were at least 3-fold lower than during iron-saturated growth; as shown with a hem1 mutant strain this regulation does not require haem synthesis. mRNA half-lives of TPI1 (TDH3) were 11.5 min (18 min) in low-iron medium and 30 min (32.5 min) in high-iron medium, indicating iron-regulation of transcript half-lives; the stabilities of the ACT1 and PDC1 transcripts were not influenced by iron. Increased glycerol production during growth in low-iron, as compared to high-iron medium, is consistent with a modification of the glycolytic flux during iron-limited growth in S. cerevisiae.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1350-0872
pubmed:author
pubmed:issnType
Print
pubmed:volume
140 ( Pt 5)
pubmed:geneSymbol
TPI1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1079-84
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Iron regulation of triosephosphate isomerase transcript stability in the yeast Saccharomyces cerevisiae.
pubmed:affiliation
Institut für Mikrobiologie, Heinrich-Heine Universität, Düsseldorf, FRG.
pubmed:publicationType
Journal Article, Comparative Study