Linked Life Data

8025634

Source:http://linkedlifedata.com/resource/pubmed/id/8025634

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1994-8-10
pubmed:abstractText
For the determination of cellular deoxynucleoside triphosphates (dNTPs) by high-performance liquid chromatography, the choice of extracting agent and periodate oxidation procedure is important for accurate results. Different extraction methods were compared using either cold methanol or trichloroacetic acid for extraction of dNTPs from lymphoblastoid cells. The recoveries of the dNTPs varied with the different extraction methods. A modification of the periodate oxidation procedure for degrading ribonucleoside triphosphates to their bases was also compared with the original method. Both methods gave accurate results when trichloroacetic acid was used as extracting agent, but when methanol was used interfering peaks were present on the chromatogram when the original method was used. These peaks were absent when the modified periodate procedure was used.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
667
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
316-21
pubmed:dateRevised
2009-1-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Comparison of extraction procedures for high-performance liquid chromatographic determination of cellular deoxynucleotides.
pubmed:affiliation
Department of Clinical Virology, Karolinska Institute, Huddinge, Sweden.
pubmed:publicationType
Journal Article, Comparative Study