| pubmed-article:8022436 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8022436 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8022436 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:8022436 | lifeskim:mentions | umls-concept:C0025920 | lld:lifeskim |
| pubmed-article:8022436 | lifeskim:mentions | umls-concept:C1510411 | lld:lifeskim |
| pubmed-article:8022436 | lifeskim:mentions | umls-concept:C0010762 | lld:lifeskim |
| pubmed-article:8022436 | lifeskim:mentions | umls-concept:C0012200 | lld:lifeskim |
| pubmed-article:8022436 | lifeskim:mentions | umls-concept:C0543482 | lld:lifeskim |
| pubmed-article:8022436 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:8022436 | lifeskim:mentions | umls-concept:C0047964 | lld:lifeskim |
| pubmed-article:8022436 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:8022436 | pubmed:dateCreated | 1994-7-29 | lld:pubmed |
| pubmed-article:8022436 | pubmed:abstractText | Transfection of specific genes into cells capable of expressing chemically induced morphological cell transformation provides a valuable approach to study the mechanisms of action of carcinogens. A human cytochrome P450 isozyme, CYP2A6, has been successfully expressed from a retroviral vector in transformable C3H/10T1/2 (10T1/2) mouse embryo fibroblasts and these resulting 10T1/2 clones were evaluated for the cytotoxic and transforming activities of two nitrosamines, 4-(methylnitrosamine)-1-(3-pyridyl)-1-butanone (NNK) and N-nitrosodiethylamine (DEN). 10T1/2 clone 29 cells, which expressed high levels of CYP2A6 activity, were responsive to the cytotoxic and morphological transforming effects of DEN or NNK on a concentration-related basis. In 10T1/2 clone 29 cells, DEN at 600 micrograms/ml decreased cell survival to 67%, and induced 0.5 type II&III foci/dish. NNK at 400 micrograms/ml administered to 10T1/2 clone 29 cells decreased survival to 57% and induced 0.43 type II&III foci/dish. Wild-type 10T1/2 cells and 10T1/2 clone 4 cells (infected with the vector but not expressing the CYP2A6 activity) were unresponsive. These results indicate that expression of a cDNA coding for cytochrome P450 in 10T1/2 cells can provide information about the role of the enzyme in the activities of chemical carcinogens and also increase the sensitivity of 10T1/2 cells to a larger number of classes of chemical carcinogens. | lld:pubmed |
| pubmed-article:8022436 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8022436 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8022436 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8022436 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8022436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8022436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8022436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8022436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8022436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8022436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8022436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8022436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8022436 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8022436 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:8022436 | pubmed:issn | 0027-5107 | lld:pubmed |
| pubmed-article:8022436 | pubmed:author | pubmed-author:HosokawaMM | lld:pubmed |
| pubmed-article:8022436 | pubmed:author | pubmed-author:LangenbachRR | lld:pubmed |
| pubmed-article:8022436 | pubmed:author | pubmed-author:NesnowSS | lld:pubmed |
| pubmed-article:8022436 | pubmed:author | pubmed-author:BeckSS | lld:pubmed |
| pubmed-article:8022436 | pubmed:author | pubmed-author:RosenblumSS | lld:pubmed |
| pubmed-article:8022436 | pubmed:author | pubmed-author:CrespiC LCL | lld:pubmed |
| pubmed-article:8022436 | pubmed:author | pubmed-author:LasleyJJ | lld:pubmed |
| pubmed-article:8022436 | pubmed:author | pubmed-author:TianoH FHF | lld:pubmed |
| pubmed-article:8022436 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8022436 | pubmed:volume | 324 | lld:pubmed |
| pubmed-article:8022436 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8022436 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8022436 | pubmed:pagination | 93-102 | lld:pubmed |
| pubmed-article:8022436 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8022436 | pubmed:meshHeading | pubmed-meshheading:8022436-... | lld:pubmed |
| pubmed-article:8022436 | pubmed:meshHeading | pubmed-meshheading:8022436-... | lld:pubmed |
| pubmed-article:8022436 | pubmed:meshHeading | pubmed-meshheading:8022436-... | lld:pubmed |
| pubmed-article:8022436 | pubmed:meshHeading | pubmed-meshheading:8022436-... | lld:pubmed |
| pubmed-article:8022436 | pubmed:meshHeading | pubmed-meshheading:8022436-... | lld:pubmed |
| pubmed-article:8022436 | pubmed:meshHeading | pubmed-meshheading:8022436-... | lld:pubmed |
| pubmed-article:8022436 | pubmed:meshHeading | pubmed-meshheading:8022436-... | lld:pubmed |
| pubmed-article:8022436 | pubmed:meshHeading | pubmed-meshheading:8022436-... | lld:pubmed |
| pubmed-article:8022436 | pubmed:meshHeading | pubmed-meshheading:8022436-... | lld:pubmed |
| pubmed-article:8022436 | pubmed:meshHeading | pubmed-meshheading:8022436-... | lld:pubmed |
| pubmed-article:8022436 | pubmed:meshHeading | pubmed-meshheading:8022436-... | lld:pubmed |
| pubmed-article:8022436 | pubmed:meshHeading | pubmed-meshheading:8022436-... | lld:pubmed |
| pubmed-article:8022436 | pubmed:meshHeading | pubmed-meshheading:8022436-... | lld:pubmed |
| pubmed-article:8022436 | pubmed:meshHeading | pubmed-meshheading:8022436-... | lld:pubmed |
| pubmed-article:8022436 | pubmed:meshHeading | pubmed-meshheading:8022436-... | lld:pubmed |
| pubmed-article:8022436 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8022436 | pubmed:articleTitle | N-nitrosodiethylamine and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone induced morphological transformation of C3H/10T1/2CL8 cells expressing human cytochrome P450 2A6. | lld:pubmed |
| pubmed-article:8022436 | pubmed:affiliation | Carcinogenesis and Metabolism Branch, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711. | lld:pubmed |
| pubmed-article:8022436 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8022436 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8022436 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
