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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1994-7-29
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pubmed:abstractText |
Transfection of specific genes into cells capable of expressing chemically induced morphological cell transformation provides a valuable approach to study the mechanisms of action of carcinogens. A human cytochrome P450 isozyme, CYP2A6, has been successfully expressed from a retroviral vector in transformable C3H/10T1/2 (10T1/2) mouse embryo fibroblasts and these resulting 10T1/2 clones were evaluated for the cytotoxic and transforming activities of two nitrosamines, 4-(methylnitrosamine)-1-(3-pyridyl)-1-butanone (NNK) and N-nitrosodiethylamine (DEN). 10T1/2 clone 29 cells, which expressed high levels of CYP2A6 activity, were responsive to the cytotoxic and morphological transforming effects of DEN or NNK on a concentration-related basis. In 10T1/2 clone 29 cells, DEN at 600 micrograms/ml decreased cell survival to 67%, and induced 0.5 type II&III foci/dish. NNK at 400 micrograms/ml administered to 10T1/2 clone 29 cells decreased survival to 57% and induced 0.43 type II&III foci/dish. Wild-type 10T1/2 cells and 10T1/2 clone 4 cells (infected with the vector but not expressing the CYP2A6 activity) were unresponsive. These results indicate that expression of a cDNA coding for cytochrome P450 in 10T1/2 cells can provide information about the role of the enzyme in the activities of chemical carcinogens and also increase the sensitivity of 10T1/2 cells to a larger number of classes of chemical carcinogens.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-(N-methyl-N-nitrosamino)-1-(3-pyri...,
http://linkedlifedata.com/resource/pubmed/chemical/Aryl Hydrocarbon Hydroxylases,
http://linkedlifedata.com/resource/pubmed/chemical/Carcinogens, Environmental,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Diethylnitrosamine,
http://linkedlifedata.com/resource/pubmed/chemical/Mixed Function Oxygenases,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrosamines,
http://linkedlifedata.com/resource/pubmed/chemical/coumarin 7-hydroxylase
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0027-5107
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
324
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
93-102
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8022436-Animals,
pubmed-meshheading:8022436-Aryl Hydrocarbon Hydroxylases,
pubmed-meshheading:8022436-Biotransformation,
pubmed-meshheading:8022436-Carcinogens, Environmental,
pubmed-meshheading:8022436-Cell Transformation, Neoplastic,
pubmed-meshheading:8022436-Cells, Cultured,
pubmed-meshheading:8022436-Clone Cells,
pubmed-meshheading:8022436-Cytochrome P-450 Enzyme System,
pubmed-meshheading:8022436-Diethylnitrosamine,
pubmed-meshheading:8022436-Fibroblasts,
pubmed-meshheading:8022436-Mice,
pubmed-meshheading:8022436-Mice, Inbred C3H,
pubmed-meshheading:8022436-Mixed Function Oxygenases,
pubmed-meshheading:8022436-Nitrosamines,
pubmed-meshheading:8022436-Transfection
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pubmed:year |
1994
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pubmed:articleTitle |
N-nitrosodiethylamine and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone induced morphological transformation of C3H/10T1/2CL8 cells expressing human cytochrome P450 2A6.
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pubmed:affiliation |
Carcinogenesis and Metabolism Branch, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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