Linked Life Data

8021938

Source:http://linkedlifedata.com/resource/pubmed/id/8021938

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-8-1
pubmed:databankReference
pubmed:abstractText
The chloroplast translational system differs from the eubacterial ones in containing several ribosomal proteins (RPs) that have no apparent homologues in eubacteria, and in having their RP genes distributed in two cellular genome compartments. The genes maintained in the organelle genome encode mainly ribosome assembly proteins. The discovery in spinach and related plants (Caryophyllidae) of a disrupted chloroplast gene encoding the ribosome assembly protein L23 raised speculations about the transfer of the functional rpl23 gene to the nucleus or the evolutionary loss of L23 protein requirement. To solve this problem, we overexpressed in E. coli the intact rpl23 gene from corn (Zea mays), purified the protein and raised antibodies. Based on immunoanalysis, we show that a prokaryotic-type L23 protein is absent in spinach. Concomittantly we have isolated a new protein from spinach chloroplast 50 S ribosomal subunits and determined its amino acid sequence. The data revealed an unexpectedly high sequence identity to the eukaryotic family of cytosolic L23 proteins (reported from yeast, trypanosome and rat), with conservation of a peptide motif responsible for the specific interaction of these proteins with domain III of 26 S and 23 S rRNA. We propose that the prokaryotic-type L23 protein in the chloroplast ribosomes of Caryophyllidae has been replaced by a homologue of the eukaryotic cytosolic L23 family. These results represent the first case of a protein (gene) substitution in chloroplast ribosome evolution, and open a new view on how the nuclear genome could progressively exert stronger control over the chloroplast translational system. We also describe experiments on the incorporation of chloroplast L23 into E. coli ribosomes, its effect on cell growth, and an unexpected immuno cross-reaction between two chloroplast RP families.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-2836
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
240
pubmed:geneSymbol
rpl23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
28-41
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:8021938-Amino Acid Sequence, pubmed-meshheading:8021938-Animals, pubmed-meshheading:8021938-Biological Evolution, pubmed-meshheading:8021938-Chloroplasts, pubmed-meshheading:8021938-Cloning, Molecular, pubmed-meshheading:8021938-Cytosol, pubmed-meshheading:8021938-Electrophoresis, Gel, Two-Dimensional, pubmed-meshheading:8021938-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:8021938-Escherichia coli, pubmed-meshheading:8021938-Escherichia coli Proteins, pubmed-meshheading:8021938-Genes, Plant, pubmed-meshheading:8021938-Molecular Sequence Data, pubmed-meshheading:8021938-Open Reading Frames, pubmed-meshheading:8021938-Organelles, pubmed-meshheading:8021938-Plant Proteins, pubmed-meshheading:8021938-Recombinant Proteins, pubmed-meshheading:8021938-Ribosomal Proteins, pubmed-meshheading:8021938-Ribosomes, pubmed-meshheading:8021938-Sequence Homology, Amino Acid, pubmed-meshheading:8021938-Vegetables, pubmed-meshheading:8021938-Zea mays
pubmed:year
1994
pubmed:articleTitle
Protein substitution in chloroplast ribosome evolution. A eukaryotic cytosolic protein has replaced its organelle homologue (L23) in spinach.
pubmed:affiliation
Max-Planck-Institut für Molekulare Genetik, Berlin, Germany.
pubmed:publicationType
Journal Article, Comparative Study