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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1994-7-29
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pubmed:databankReference |
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pubmed:abstractText |
The human c-mpl proto-oncogene encodes a member of the cytokine receptor superfamily, expressed mainly in CD 34-positive hematopoietic progenitors and in the megakaryocytic lineage. To investigate the elements required for this tissue-specific expression, we cloned the human c-mpl gene (MPL) as well as the 5' end of the mouse gene. The human c-mpl gene contains 12 exons distributed over 17 kb of DNA. Each of the two "cytokine receptor domains" of Mpl is encoded by a set of four exons, the transmembrane domain by a single exon and the cytoplasmic domain by two exons. We also describe how three types of mRNA, encoding different proteins, are generated. The major species contains all 12 exons; mRNAs encoding a protein with a smaller cytoplasmic domain are produced by termination of the transcript within intron 10, and mRNAs encoding a putative soluble form of the c-Mpl protein lack exons 9 and 10. The promoter regions of the human and mouse genes were characterized. These promoters are GC-rich and contain putative binding sites for proteins of the Ets and GATA families. Finally, we show that a 700-bp fragment of the human c-mpl promoter is active in the HEL and K562 cell lines, which express erythroid and megakaryocytic markers, but is inactive in the nonhematopoietic HeLa cell line and the Jurkat T lymphoid cell line.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/MPL protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytokine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Thrombopoietin
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0888-7543
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
20
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pubmed:geneSymbol |
c-mpl
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5-12
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8020956-Amino Acid Sequence,
pubmed-meshheading:8020956-Animals,
pubmed-meshheading:8020956-Base Sequence,
pubmed-meshheading:8020956-Cell Line,
pubmed-meshheading:8020956-Cloning, Molecular,
pubmed-meshheading:8020956-DNA, Complementary,
pubmed-meshheading:8020956-Exons,
pubmed-meshheading:8020956-Gene Expression,
pubmed-meshheading:8020956-Hematopoiesis,
pubmed-meshheading:8020956-Humans,
pubmed-meshheading:8020956-Introns,
pubmed-meshheading:8020956-Mice,
pubmed-meshheading:8020956-Molecular Sequence Data,
pubmed-meshheading:8020956-Neoplasm Proteins,
pubmed-meshheading:8020956-Promoter Regions, Genetic,
pubmed-meshheading:8020956-Proto-Oncogene Proteins,
pubmed-meshheading:8020956-Proto-Oncogenes,
pubmed-meshheading:8020956-RNA, Messenger,
pubmed-meshheading:8020956-Receptors, Cytokine,
pubmed-meshheading:8020956-Receptors, Immunologic,
pubmed-meshheading:8020956-Receptors, Thrombopoietin,
pubmed-meshheading:8020956-Transcription, Genetic
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pubmed:year |
1994
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pubmed:articleTitle |
Structure and transcription of the human c-mpl gene (MPL).
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pubmed:affiliation |
Institut National de la Santé et de la Recherche Médicale, U. 91, Hôpital Henri Mondor, Creteil, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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