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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1994-8-4
|
| pubmed:abstractText |
Boar transition protein 1 was extracted with acid from the testes, purified by chromatographies on CM-Sephadex C-25 and Sephadex G-50, and reduced and carboxymethylated. The modified protein was purified by HPLC on Nucleosil 300 7C18. The primary structure of the protein was determined by automated Edman degradation of the C-terminal peptide of the BrCN-cleaved protein and of the whole protein, and by carboxypeptidase digestion of it. The study of phosphorylation sites showed that Ser36 and Ser39 in the very conserved sequence 29-42 were partly phosphorylated, suggesting the involvement of this region in the interaction with DNA.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
1039-9712
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
32
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
349-57
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8019440-Amino Acid Sequence,
pubmed-meshheading:8019440-Animals,
pubmed-meshheading:8019440-Binding Sites,
pubmed-meshheading:8019440-Chromatography, Ion Exchange,
pubmed-meshheading:8019440-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:8019440-Male,
pubmed-meshheading:8019440-Molecular Sequence Data,
pubmed-meshheading:8019440-Phosphorylation,
pubmed-meshheading:8019440-Swine,
pubmed-meshheading:8019440-Testis
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
The amino acid sequence and phosphorylation sites of a boar transition protein 1.
|
| pubmed:affiliation |
Department of Chemistry, Faculty of Science, Chiba University, Japan.
|
| pubmed:publicationType |
Journal Article
|