Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1994-7-25
pubmed:abstractText
Three pairs of oligonucleotide primers specific for different regions of the hrp gene (hypersensitive reaction and pathogenicity) cluster of Xanthomonas campestris pv. vesicatoria were designed and tested for amplification of DNA isolated from a large number of different bacteria. DNA sequences related to the hrp genes were successfully amplified from X. fragariae and from 28 pathovars of X. campestris. No DNA amplification occurred with genomic DNA from phytopathogenic strains of X. campestris pv. secalis, X. campestris pv. translucens, and X. albilineans or from nonpathogenic opportunistic xanthomonads and phytopathogenic strains of the genera Acidovorax, Agrobacterium, Clavibacter, Erwinia, Pseudomonas, and Xylella. The DNA from those bacteria also failed to hybridize to hrp-specific fragments in Southern blot analysis. DNA fragments amplified with a particular primer pair were of identical size from each of the different phytopathogenic xanthomonads. However, restriction analysis of these fragments by using frequently cutting endonucleases revealed variation in the pattern for these hrp-related fragments amplified from the different Xanthomonas strains. The restriction patterns generated for the different fragments allowed distinction of the strains representing a pathovar or species of phytopathogenic xanthomonads. We believe that DNA amplification with hrp-specific oligonucleotide primers is a highly sensitive and specific method that can be applied for detection and identification of phytopathogenic xanthomonads.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-1312532, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-1370664, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-1472716, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-1472717, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-1482178, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-1482193, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-16348131, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-1741613, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-1768118, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-2275852, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-2824440, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-2824447, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-3023280, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-3190225, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-377280, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-6312838, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-7012838, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-7198910, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-7912499, http://linkedlifedata.com/resource/pubmed/commentcorrection/8017904-8476288
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
60
pubmed:geneSymbol
hrp, hrpA, hrpB, hrpC, hrpD, hrpE, hrpF
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1068-77
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Detection and identification of phytopathogenic Xanthomonas strains by amplification of DNA sequences related to the hrp genes of Xanthomonas campestris pv. vesicatoria.
pubmed:affiliation
Department of Plant Pathology, University of Florida, Gainesville 32611.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't