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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1994-7-28
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pubmed:databankReference |
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pubmed:abstractText |
In a search for trypanosome DNA sequences that permit replication and stable maintenance of extrachromosomal elements, a 1-kilobase-pair (kbp) fragment from a mitochondrial kinetoplast DNA (kDNA) minicircle of Trypanosoma brucei was isolated and characterized. The plasmid pTbo-1, carrying the kDNA element, is maintained in T. brucei as a supercoiled concatemer containing approximately seven to nine pTbo-1 monomer units (5.6 kbp each) in a head-to-tail orientation. The concatemer is found in approximately one copy per cell when procyclic trypanosomes are cultured in the presence of 100 micrograms of hygromycin per ml; however, in the absence of continuous hygromycin selection, the plasmid is lost from the population with a t1/2 of approximately 8.7 days (17 cell generations). A second unrelated kDNA minicircle was also able to serve as an autonomously replicating sequence (ARS) element in T. brucei, suggesting that this is a general property of kDNA minicircles. Replication of mitochondrial DNA in the nucleus may be due to either a specific consensus sequence (such as in yeast ARS elements) or nonspecific sequence characteristics (such as the degree of A&T-richness or bent DNA).
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-1323120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-1324122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-1688737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-1745242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-1840521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-1876188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-2001590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-2006150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-2009863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-2142018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-2191298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-2300209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-2304458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-2452697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-2499047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-2542768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-2674674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-2995831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-3021587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-3043195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-3082787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-3182827,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-3786134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-438220,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-8386315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-8441667,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8016097-881656
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5962-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8016097-Animals,
pubmed-meshheading:8016097-Base Sequence,
pubmed-meshheading:8016097-Blotting, Southern,
pubmed-meshheading:8016097-DNA, Kinetoplast,
pubmed-meshheading:8016097-DNA, Mitochondrial,
pubmed-meshheading:8016097-DNA, Protozoan,
pubmed-meshheading:8016097-DNA, Superhelical,
pubmed-meshheading:8016097-DNA Primers,
pubmed-meshheading:8016097-DNA Replication,
pubmed-meshheading:8016097-Kinetics,
pubmed-meshheading:8016097-Microscopy, Electron,
pubmed-meshheading:8016097-Molecular Sequence Data,
pubmed-meshheading:8016097-Plasmids,
pubmed-meshheading:8016097-Restriction Mapping,
pubmed-meshheading:8016097-Transfection,
pubmed-meshheading:8016097-Trypanosoma brucei brucei
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pubmed:year |
1994
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pubmed:articleTitle |
Mitochondrial minicircle DNA supports plasmid replication and maintenance in nuclei of Trypanosoma brucei.
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pubmed:affiliation |
Intercampus Program in Molecular Parasitology, University of California, San Francisco 94143-1204.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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