| pubmed-article:8012612 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8012612 | lifeskim:mentions | umls-concept:C1709696 | lld:lifeskim |
| pubmed-article:8012612 | lifeskim:mentions | umls-concept:C0995763 | lld:lifeskim |
| pubmed-article:8012612 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:8012612 | lifeskim:mentions | umls-concept:C0066672 | lld:lifeskim |
| pubmed-article:8012612 | pubmed:dateCreated | 1994-7-25 | lld:pubmed |
| pubmed-article:8012612 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8012612 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8012612 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8012612 | pubmed:abstractText | We have identified, cloned and sequenced three tuf-like genes from Streptomyces ramocissimus (Sr.), the producer of the antibiotic kirromycin which inhibits protein synthesis by binding the polypeptide chain elongation factor EF-Tu. The tuf-1 gene encodes a protein with 71% amino acid residues identical to the well characterized elongation factor Tu of Escherichia coli (Ec.EF-Tu). The genetic location of tuf-1 downstream of a fus homologue and the in vitro activity of Sr.EF-Tu1 show that tuf-1 encodes a genuine EF-Tu. The putative Sr.EF-Tu2 and Sr.EF-Tu3 proteins are 69% and 63% identical to Ec.EF-Tu. Homologues of tuf-1 and tuf-3 were detected in all five Streptomyces strains investigated, but tuf-2 was found in S. ramocissimus only. The three tuf genes were expressed in E. coli and used to produce polyclonal antibodies. Western blot analysis showed that Sr.EF-Tu1 was present at all times under kirromycin production conditions in submerged and surface-grown cultures of S. ramocissimus and in germinating spores. The expression of tuf-2 and tuf-3 was, however, below the detection level. Surprisingly, Sr.EF-Tu1 was kirromycin sensitive, which excludes the possibility that EF-Tu is involved in the kirromycin resistance of S. ramocissimus. | lld:pubmed |
| pubmed-article:8012612 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8012612 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8012612 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8012612 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8012612 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8012612 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:8012612 | pubmed:issn | 1350-0872 | lld:pubmed |
| pubmed-article:8012612 | pubmed:author | pubmed-author:BoschLL | lld:pubmed |
| pubmed-article:8012612 | pubmed:author | pubmed-author:RietveldKK | lld:pubmed |
| pubmed-article:8012612 | pubmed:author | pubmed-author:ShochatSS | lld:pubmed |
| pubmed-article:8012612 | pubmed:author | pubmed-author:VijgenboomEE | lld:pubmed |
| pubmed-article:8012612 | pubmed:author | pubmed-author:van WezelG... | lld:pubmed |
| pubmed-article:8012612 | pubmed:author | pubmed-author:HeinstraP WPW | lld:pubmed |
| pubmed-article:8012612 | pubmed:author | pubmed-author:WoudtL PLP | lld:pubmed |
| pubmed-article:8012612 | pubmed:author | pubmed-author:van HaarlemJJ | lld:pubmed |
| pubmed-article:8012612 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8012612 | pubmed:volume | 140 ( Pt 4) | lld:pubmed |
| pubmed-article:8012612 | pubmed:geneSymbol | tuf | lld:pubmed |
| pubmed-article:8012612 | pubmed:geneSymbol | tuf-2 | lld:pubmed |
| pubmed-article:8012612 | pubmed:geneSymbol | tuf-1 | lld:pubmed |
| pubmed-article:8012612 | pubmed:geneSymbol | tuf-3 | lld:pubmed |
| pubmed-article:8012612 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8012612 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8012612 | pubmed:pagination | 983-98 | lld:pubmed |
| pubmed-article:8012612 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:8012612 | pubmed:meshHeading | pubmed-meshheading:8012612-... | lld:pubmed |
| pubmed-article:8012612 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8012612 | pubmed:articleTitle | Three tuf-like genes in the kirromycin producer Streptomyces ramocissimus. | lld:pubmed |
| pubmed-article:8012612 | pubmed:affiliation | Department of Biochemistry, Leiden University, Gorlaeus Laboratories, The Netherlands. | lld:pubmed |
| pubmed-article:8012612 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8012612 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:8012612 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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