Linked Life Data

8012612

Source:http://linkedlifedata.com/resource/pubmed/id/8012612

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-7-25
pubmed:databankReference
pubmed:abstractText
We have identified, cloned and sequenced three tuf-like genes from Streptomyces ramocissimus (Sr.), the producer of the antibiotic kirromycin which inhibits protein synthesis by binding the polypeptide chain elongation factor EF-Tu. The tuf-1 gene encodes a protein with 71% amino acid residues identical to the well characterized elongation factor Tu of Escherichia coli (Ec.EF-Tu). The genetic location of tuf-1 downstream of a fus homologue and the in vitro activity of Sr.EF-Tu1 show that tuf-1 encodes a genuine EF-Tu. The putative Sr.EF-Tu2 and Sr.EF-Tu3 proteins are 69% and 63% identical to Ec.EF-Tu. Homologues of tuf-1 and tuf-3 were detected in all five Streptomyces strains investigated, but tuf-2 was found in S. ramocissimus only. The three tuf genes were expressed in E. coli and used to produce polyclonal antibodies. Western blot analysis showed that Sr.EF-Tu1 was present at all times under kirromycin production conditions in submerged and surface-grown cultures of S. ramocissimus and in germinating spores. The expression of tuf-2 and tuf-3 was, however, below the detection level. Surprisingly, Sr.EF-Tu1 was kirromycin sensitive, which excludes the possibility that EF-Tu is involved in the kirromycin resistance of S. ramocissimus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1350-0872
pubmed:author
pubmed:issnType
Print
pubmed:volume
140 ( Pt 4)
pubmed:geneSymbol
tuf, tuf-1, tuf-2, tuf-3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
983-98
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8012612-Amino Acid Sequence, pubmed-meshheading:8012612-Bacterial Proteins, pubmed-meshheading:8012612-Base Sequence, pubmed-meshheading:8012612-Cloning, Molecular, pubmed-meshheading:8012612-Drug Resistance, Microbial, pubmed-meshheading:8012612-Escherichia coli, pubmed-meshheading:8012612-Gene Expression Regulation, pubmed-meshheading:8012612-Genes, Bacterial, pubmed-meshheading:8012612-Molecular Sequence Data, pubmed-meshheading:8012612-Peptide Elongation Factor Tu, pubmed-meshheading:8012612-Pyridones, pubmed-meshheading:8012612-Recombinant Fusion Proteins, pubmed-meshheading:8012612-Sequence Alignment, pubmed-meshheading:8012612-Sequence Homology, pubmed-meshheading:8012612-Species Specificity, pubmed-meshheading:8012612-Spores, Bacterial, pubmed-meshheading:8012612-Streptomyces
pubmed:year
1994
pubmed:articleTitle
Three tuf-like genes in the kirromycin producer Streptomyces ramocissimus.
pubmed:affiliation
Department of Biochemistry, Leiden University, Gorlaeus Laboratories, The Netherlands.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't