Linked Life Data

8011783

Source:http://linkedlifedata.com/resource/pubmed/id/8011783

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-7-26
pubmed:abstractText
Several reports have shown an association between lameness in cattle and vaccination with Brucella abortus strain 19. Affected joints are culture negative for Brucella, but the synovial fluid is positive for B. abortus antibodies. The joints contain cloudy fluid, with villous proliferation of the synovium. Brucella abortus antigens are often found in the synovium with fluorescent antibody staining. This report describes the experimental reproduction of a chronic synovitis in 6 young Angus steers using intra-articular injections of B. abortus strain 19. The carpal and tibial joints were injected with 5 x 10(9) colony-forming units/ml of B. abortus strain 19 and regularly biopsied over a 28-day period. Steers started becoming serologically positive for B. abortus on post-inoculation day (PID) 5 and were all positive by PID 7. Joints were cultured and examined by fluorescent antibody staining, immunohistochemical methods, and light and transmission electron microscopy. Lesions typical of the field cases were present by PID 21. Brucella abortus was cultured more often during PID 1-5 (6 of 9 joints) than during PID 7-28 (3 of 15 joints). Brucella abortus was only found on PID 1 and 5 by fluorescent antibody staining and in only 2 joints immunohistochemically on PID 5 and 7. The reproduction of lesions typical of field cases but the inability to locate B. abortus antigens in the synovium raises the question of whether in field cases the synovium is continually or intermittently seeded with bacteria or if factors other than just the bacterium are needed to perpetuate the lesion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1040-6387
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
56-61
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Experimental Brucella abortus strain 19 arthritis in young cattle.
pubmed:affiliation
California Veterinary Diagnostic Laboratory, Davis 95617.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't